FastQCFastQC Report
Wed 25 Feb 2015
VE06_GGACTCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameVE06_GGACTCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5281160
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT11100.012.3646314
TCTATAC10050.011.8654473
TACACCG7550.011.6222965
GTATTAG10100.010.9216331
TACACTG14550.010.8245385
GTCTTAC5500.010.6420061
ACCGTGC7850.010.6048958
GTGCCAG9250.010.4650081
GTATAGG7750.010.1666811
GCTACAC6200.010.1610983
GTGTTAG7550.010.1378251
GTGTGTA13600.010.0972651
ACTATAC10800.09.9998113
ACTGTGC12200.09.9588288
ACTGTCC9050.09.9445638
GTCTTAG6150.09.8832891
CCCCTGG3653.6938218E-89.8682451
ACACCGT7550.09.834256
GTGCTAC7850.09.7503931
TAGACAG9000.09.7498155