FastQCFastQC Report
Wed 25 Feb 2015
VE05_TCCTGAGC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameVE05_TCCTGAGC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9957634
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC129950.012.50149245
ACTGTGC15250.012.0982678
CTATACT15100.011.3244154
CAGGAGT14750.011.2880474
TAGACAG14050.011.2098775
TCTATAC14050.011.2098773
CTTATAC228650.011.0413645
TTATACA191500.011.03310645
GTATAGG11550.010.9090051
TATACTG23400.010.86535
TACACTG20400.010.8087395
CTGTCCC11350.010.70476159
GTATTAG13900.010.6833711
TATACAG18050.010.4708325
AGGACCG7100.010.4576655
ATACACA108800.010.402554545
TTACACT22500.010.3999194
TACACAG14750.010.37285
GTGTAGG11950.010.3555681
TATACCG11950.010.3555685
Produced by FastQC (version 0.11.2)