FastQCFastQC Report
Wed 25 Feb 2015
VE05_TCCTGAGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameVE05_TCCTGAGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9957634
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG14050.013.2916025
TACACTG18350.012.8744535
TACACAG15100.012.6653595
TATACAC132350.012.34275745
CTATACT14850.012.1210174
TATACAG17050.011.8766425
CCTACAC13700.011.8246273
CTACACT18900.011.7855244
TACACCG14200.011.7251635
TCTATAC13700.011.1677043
CTAATAC14000.011.08910753
CTTATAC231400.010.99762245
CCTATAC12900.010.9881963
TTATACA191700.010.95113345
GTCCTAC6200.010.8921731
TCTAGAC15700.010.8915443
ACTGTGC17950.010.6544268
ACTGTCC12500.010.6198298
CTAGACA15450.010.4852694
GTAGCAC12150.010.3702043
Produced by FastQC (version 0.11.2)