FastQCFastQC Report
Wed 25 Feb 2015
VE04_AGGCAGAA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameVE04_AGGCAGAA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9017218
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC358100.013.91138845
TAGACAG7800.013.5576245
ATACACA253950.012.91833345
TATACCG8650.012.4854855
TTATACA498050.011.97209545
CTACACC12950.011.8146124
TACACAT181100.011.6914545
TAAGAGT7400.011.5539964
CCTACAC8800.011.5056243
TATACTG12950.011.4671235
TACACCG13300.011.3345295
ACTGTGC10750.011.0929678
TATACAG9800.011.0203525
GTATAGA10650.010.9858611
TCTGACG44000.010.94355145
CTATACT8100.010.8332794
AGGACCG10050.010.7462155
ACACATC132450.010.70251345
TTAGTAC8700.010.60339453
GTGTAGA16450.010.3950841
Produced by FastQC (version 0.11.2)