FastQCFastQC Report
Wed 25 Feb 2015
VE04_AGGCAGAA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameVE04_AGGCAGAA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9017218
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC28400.014.7361945
TATACAC362050.013.90855845
CTCCGAG32750.013.12238245
CTATACT8600.013.08120254
ATACACA257150.012.94113245
TCTATAC9500.012.7892863
TATACCG7450.012.6843785
GTATAAG18150.012.64451315
TATACTG12800.012.1287295
TTATACA502500.012.076344545
TACACCG13050.011.8963785
GTATAGA11550.011.8882821
TACACTG10850.011.8201045
GTGTATA19950.011.72921613
TACACAT182250.011.69157545
TAGACAG8550.011.5787775
CTAACAC7250.011.482593
CTACACT10200.011.4704214
GTGTAGG8850.011.4456511
TAGGACC4201.8189894E-1211.2498354
Produced by FastQC (version 0.11.2)