FastQCFastQC Report
Wed 25 Feb 2015
VE03_CTCTCTAC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameVE03_CTCTCTAC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4808947
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCC8100.015.555529
CTAGCAC5100.014.9999663
TACACTG14200.014.8943335
CTAGGAC4900.014.6938443
TGTCCCA12650.014.58494710
CTACACT14350.014.4250534
CTTACAC8150.014.0797213
TCTATAC8650.014.046213
TATACAG13500.013.9999675
GTCCAGG4700.013.8829471
CGTATAC6100.013.6475093
GCTGTCC7950.013.5848748
CTATACT10150.013.5221364
TCTACAC14050.012.97153
TAGGGCG2451.6212653E-812.8571135
TAGACTG7050.012.7659285
ATACCGC4950.012.7272436
ACAGTGC12950.012.6833688
CAGGCTG7800.012.4038175
CGTGGGC3457.2759576E-1212.39127513
Produced by FastQC (version 0.11.2)