FastQCFastQC Report
Wed 25 Feb 2015
VE03_CTCTCTAC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameVE03_CTCTCTAC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4808947
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG18800.015.0795585
TCTACAC16750.014.9102213
TACACCG6950.014.8918595
TATACCG6450.014.6509395
GTGTAGA16300.014.5004281
GTAGGAC4200.014.4640663
TACACAG12350.014.2103095
AGGACAG5850.013.84594255
CTACACT16950.013.6723584
CTACACA14700.013.6222414
GTCCTAC4150.013.5603541
ACTGTGC11350.013.4799718
CTATACT9250.013.3781754
CTAGCAC5400.013.333133
GTATAGA9350.013.0005371
GTGTTAG8500.012.9764621
TACACGG5600.012.8569475
CCTACAC11600.012.8015283
TAGACTG7400.012.7700765
GTATAAG9000.012.7557731
Produced by FastQC (version 0.11.2)