FastQCFastQC Report
Wed 25 Feb 2015
VE01_TAAGGCGA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameVE01_TAAGGCGA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10969799
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACAC14550.012.2164643
TATACAC92800.012.12314545
ACCGCTC14700.012.0918058
GTGCTAC16400.011.93594551
TACACTG20900.011.5191095
GTAGCAC17150.010.4956013
GTATTAG20000.010.4624741
CCGCTCC14350.010.3484059
GTGTAGC16600.010.1656371
CTACACC21800.010.1146544
ACAGTGC20700.09.8912798
TTATACA143500.09.76853545
GTCTAGA15700.09.7451981
TAGACAG16200.09.72219855
TTAAGGC13500.09.6666423
CCTAGGG5600.09.6428332
GTAGGAC7700.09.6428333
CTAGTAC14500.09.6206663
CTTATAC155700.09.58116345
GTATTGG28950.09.4818411
Produced by FastQC (version 0.11.2)