FastQCFastQC Report
Wed 25 Feb 2015
VE01_TAAGGCGA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameVE01_TAAGGCGA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10969799
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAC17200.013.7413221
ACCGCTC14350.012.5433558
TATACAC92150.012.330749545
GTGTAGC16100.012.0237581
GCTACAC16700.011.7213713
GTAGCAC17150.011.28261853
TCTATAC17500.010.9283973
TACACCA27350.010.7767945
CTACACC22600.010.7520414
TACACTG21600.010.7289955
ACAGTGC22050.010.6120778
CTAGCAC11500.010.36943
GTATAGA23100.010.3290791
TTATACA140950.010.21664445
TACACCG11850.010.0631315
TATACTG28250.09.9555945
CTAAGAC13000.09.8652283
ACCACGT20800.09.7354228
AGCACCG23150.09.7190685
GCACCGC26900.09.7024486
Produced by FastQC (version 0.11.2)