FastQCFastQC Report
Fri 13 Feb 2015
lane2_Undetermined_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69246504
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC1193700.012.61411345
TCGTGGG98750.010.82102512
TTATACA1688300.010.76511545
TACACCG91500.010.5223395
ATACACA949850.010.16074545
TACACTG155950.010.1549285
CTTATAC2001850.010.05714345
CTATACT109550.09.7741754
ACTGTGC121500.09.7389378
TATACTG179250.09.5628075
ACTGTCC104950.09.5170578
GTGTAGG109850.09.3788591
CTTACAC99200.09.342673
CTACACC131700.09.2917744
GTGTAGC107100.09.2416121
CTACACT152000.09.1607824
GTGCTAC96300.08.9232221
GTTCTAG119150.08.8733651
TATACAG159750.08.8713415
ACAGTGC141600.08.8331118
Produced by FastQC (version 0.11.2)