FastQCFastQC Report
Fri 13 Feb 2015
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69246504
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC1212400.012.61898945
CTCGTAT128350.011.21759
TATGCCG132000.010.90759513
TTATACA1699200.010.84642245
TACACCG94850.010.3646845
TACACTG150650.010.3186035
ATACACA955750.010.2894245
CTATACT113350.010.1417144
CTTATAC2027250.010.0749945
GCCGTCT154700.09.9473116
GTATGCC150600.09.64982712
GTATTAG103350.09.64751
ACTGTGC121000.09.5934838
GTGTAGG104650.09.5706691
TCGTATG152100.09.53987110
CTCCGAG171950.09.49049945
TATACTG181850.09.4636815
GTGCTAC103200.09.4434291
TCCGAGC133650.09.33026645
GTAGCAC103950.09.3274893
Produced by FastQC (version 0.11.2)