FastQCFastQC Report
Fri 13 Feb 2015
lane1_Undetermined_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5108952
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACG6800.029.3241441
CTCTACG16450.024.107471
ACTGGAG35000.023.5969410
GGAGTTC26050.023.14975213
GTGACTG22350.022.7408547
GACTGGA35650.022.4714499
CGGGTGA6050.022.302126
GGACGGG10650.022.1099683
CGGGAGT6050.021.20201511
TGACTGG25250.020.9423648
CTGGAGT27250.019.90252711
AGACTGG12750.019.5898938
ACGGGAG9100.019.53634810
CTACGTG8700.019.3326053
TCTACGT12350.019.0554712
TGGAGTT30000.018.6026712
GACAGGA22500.018.4024629
GGGACGG13300.018.199922
GAGTTCA31600.018.1617514
CTGATCG8650.018.1311871
Produced by FastQC (version 0.11.2)