FastQCFastQC Report
Fri 13 Feb 2015
lane1_Undetermined_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5108952
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTCC16850.014.6670061
AGCTTTG18450.014.2474821
CCCGGAC7200.012.79807421
CCCGAGC7750.012.4656641
AGCTTCG10950.012.3107861
GCCCGGA7750.011.60039520
AGCTTAG5550.011.3347951
CCGGACA8950.011.05116322
AGCTTAC12250.011.0043341
TCCCGAG6150.010.5912745
AGCTTCA21600.010.4014741
AGCTTAT17500.010.2707131
CGGACAG9100.010.12793323
AGCTTTT32650.09.977771
AGCTTGT15000.09.8855611
CTTCCCG8500.09.2485693
AGCTTTC19750.08.9868741
CCGAGCG11150.08.8638052
GCTTCCC11850.08.5297552
GGACAGT12400.08.34154124
Produced by FastQC (version 0.11.2)