FastQCFastQC Report
Fri 13 Feb 2015
SE379_CAGATC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE379_CAGATC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15605330
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC14850.013.63682843
GTAGGGA19300.011.54136418
TGGTCGC17550.011.28243642
GTGGTCG18750.011.28038441
AGCGTCG20400.011.13962210
GAGCGTC20250.010.6665879
GCGTCGT21250.010.37639211
CGTGTAG22000.09.9203815
GTCGCCG21300.09.71864144
AGAGCGT24000.09.2811798
CGTCGTG24600.08.963346512
TGTAGGG25250.08.91082417
AGACGTG24150.08.66464120
CTCGGTG26800.08.563395537
TCGCCGT24550.08.52371245
GGTGGTC25550.08.45402940
CGGTGGT26550.08.38979439
GTGTAGG25800.08.37202916
TAGGGAA31200.08.0048519
GGTGACT18900.07.976136
Produced by FastQC (version 0.11.2)