FastQCFastQC Report
Fri 13 Feb 2015
SE379_CAGATC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE379_CAGATC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15605330
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19900.012.32462744
GTATGCC21800.011.45689345
GCACACG25400.09.92109611
ACGTCTG26250.09.51412815
TCGTATG25650.09.47352843
AGTCACC24300.09.35169628
ACACGTC28000.08.91949513
CACGTCT28850.08.81267914
CACACGT27950.08.77445112
CTCGTAT26400.08.43738742
CCAGATC32000.07.94518133
CAGTCAC30050.07.861817427
CGTCTGA33400.07.74687716
ACCAGAT34950.07.596440332
ACTCCAG35500.07.54220123
CCAGTCA34700.07.52153826
GTCACCA32800.07.40841429
TCACCAG38200.06.95014630
GAACTCC37850.06.71725821
TCCAGTC40150.06.50055625
Produced by FastQC (version 0.11.2)