FastQCFastQC Report
Fri 13 Feb 2015
SE378_TGACCA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE378_TGACCA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8880933
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT330550.37220188464432735Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA51850.037.92650218
GGTCGCC49100.037.7603243
TGGTCGC49950.036.9375742
GAGCGTC52500.036.8997969
GCGTCGT51900.036.67609811
GTGGTCG51850.036.45192341
CGTGTAG52950.036.20376615
AGCGTCG53800.036.0918110
GTCGCCG51900.035.89676744
AGAGCGT55250.035.877638
GTGTAGG54800.035.72060416
CTCGGTG54750.035.26008237
CGTCGTG54350.035.14712
TGTAGGG56150.034.82171617
AAGAGCG59000.034.512527
CGGTGGT55250.034.4930239
TCGCCGT49500.034.1825945
GATCTCG58250.033.8755434
GAGTGTA59200.033.71180727
GGTGGTC57000.033.43421640
Produced by FastQC (version 0.11.2)