FastQCFastQC Report
Fri 13 Feb 2015
SE378_TGACCA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE378_TGACCA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8880933
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC378780.42650924176547667TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC55950.037.4002444
GTATGCC57300.036.4012845
GCACACG59850.035.6010411
ACGTCTG60050.035.59487515
ACACGTC60000.035.5120413
TCGTATG59450.035.23503543
CACACGT61000.034.9667612
CACGTCT61000.034.9667614
CTCGTAT57950.034.2445942
CAGTCAC61950.034.139827
CGTCTGA62900.034.0178416
CCAGTCA63150.033.49106226
ACTGACC63100.033.4106332
CTGACCA62950.033.20430433
ACTCCAG65100.032.66068323
GAACTCC65750.032.2351421
GTCACTG66450.031.79399129
TCCAGTC66400.031.7501625
CACTGAC68400.030.9533731
AGCACAC69800.030.65487110
Produced by FastQC (version 0.11.2)