FastQCFastQC Report
Fri 13 Feb 2015
SE377_GCCAAT_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE377_GCCAAT_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9668553
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT688700.7123092773034393Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC89050.041.1360743
GTAGGGA95950.041.01329818
GAGCGTC96550.040.7351239
TGGTCGC91500.040.18216342
CGTGTAG94900.040.11566515
GCGTCGT96050.039.9398911
GTGGTCG95600.039.65923741
AGAGCGT100700.039.5255788
AGCGTCG100200.039.3410810
GTGTAGG99750.038.81931316
TAGGGAA101100.038.8128219
GTCGCCG94500.038.81129544
GATCTCG102400.038.71558834
CTCGGTG101750.038.63121437
AAGAGCG105600.038.54387
CGTCGTG100150.038.25987612
TGTAGGG101200.038.21864317
ATCTCGG104450.038.10651835
TCGCCGT89100.037.6027345
CGGTGGT102850.037.51839