Basic Statistics
Measure | Value |
---|---|
Filename | SE377_GCCAAT_L002_R2_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9668553 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT | 68870 | 0.7123092773034393 | Illumina Single End PCR Primer 1 (100% over 51bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTCGCC | 8905 | 0.0 | 41.13607 | 43 |
GTAGGGA | 9595 | 0.0 | 41.013298 | 18 |
GAGCGTC | 9655 | 0.0 | 40.735123 | 9 |
TGGTCGC | 9150 | 0.0 | 40.182163 | 42 |
CGTGTAG | 9490 | 0.0 | 40.115665 | 15 |
GCGTCGT | 9605 | 0.0 | 39.93989 | 11 |
GTGGTCG | 9560 | 0.0 | 39.659237 | 41 |
AGAGCGT | 10070 | 0.0 | 39.525578 | 8 |
AGCGTCG | 10020 | 0.0 | 39.34108 | 10 |
GTGTAGG | 9975 | 0.0 | 38.819313 | 16 |
TAGGGAA | 10110 | 0.0 | 38.81282 | 19 |
GTCGCCG | 9450 | 0.0 | 38.811295 | 44 |
GATCTCG | 10240 | 0.0 | 38.715588 | 34 |
CTCGGTG | 10175 | 0.0 | 38.631214 | 37 |
AAGAGCG | 10560 | 0.0 | 38.5438 | 7 |
CGTCGTG | 10015 | 0.0 | 38.259876 | 12 |
TGTAGGG | 10120 | 0.0 | 38.218643 | 17 |
ATCTCGG | 10445 | 0.0 | 38.106518 | 35 |
TCGCCGT | 8910 | 0.0 | 37.60273 | 45 |
CGGTGGT | 10285 | 0.0 | 37.518 | 39 |