FastQCFastQC Report
Fri 13 Feb 2015
SE377_GCCAAT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE377_GCCAAT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9668553
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC825250.8535403384560233TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC103150.041.05123529
AGTCACG106900.039.94793728
CGTATGC106400.039.71417244
GCACACG109550.039.70043611
TCACGCC106150.039.6790930
ACGTCTG109500.039.55418415
CACACGT110650.039.24476212
GTATGCC107550.039.20583745
CACGCCA108100.038.9633231
ACACGTC111800.038.88133213
CTCGTAT107050.038.3998942
TCGTATG110700.038.37359243
CACGTCT113400.038.3525914
CGTCTGA114650.037.7381916
GAACTCC116350.036.9160621
CGCCAAT114500.036.8837133
ACTCCAG116200.036.7700923
CCAGTCA116500.036.5402126
GATCGGA120550.036.411321
TCCAGTC117300.036.3869125
Produced by FastQC (version 0.11.2)