FastQCFastQC Report
Fri 13 Feb 2015
MRP31_ACTTGA_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP31_ACTTGA_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5082669
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAGC10150.020.3922121
CCCGGAC10750.019.46543121
GCCCGGA11150.018.56532320
TGCCCGG11400.017.96081519
CCGAGCG11700.017.4978732
CGGACAG12600.016.78433623
CGAGCGT13000.015.5751833
CCGGACA13450.015.55664722
TTGCCCG13400.015.28009818
GAGCGTG13800.014.8348614
GCGTGAA14850.014.3909156
ATGCGCC10900.013.21044310
ATGGCCC11150.012.71433540
TGCGCCT11250.012.59946111
AGCTTCC17100.012.4988491
GGACAGT16950.012.47820124
GAGCAGT17600.012.40211141
AGCTTTC19550.012.1983651
AGCTTAC9050.012.1811991
AGCTTTG20350.012.1610431
Produced by FastQC (version 0.11.2)