FastQCFastQC Report
Fri 13 Feb 2015
MRP31_ACTTGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP31_ACTTGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5082669
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGCGTGAAAGGATTTGCCCGGACAGTTTGCTTCATGGAGCAGTTTTT52880.10403982631959706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCG12500.019.9808352
CCCGAGC12550.019.9057331
CCCGGAC12650.019.74215921
GCCCGGA12850.019.43488920
TGCCCGG13200.018.74912319
CGGACAG13950.017.90238823
GAGCGTG15150.016.9305674
CGAGCGT15100.016.8376243
CCGGACA15000.016.79921522
TTGCCCG15750.015.99925118
GCGTGAA17050.014.7799496
AGCTTAC9100.013.8498671
GGACAGT18750.013.68030124
GAGCAGT19700.013.4777241
AGCGTGA20450.012.7627415
ATGGAGC20450.012.54343438
GGAGCAG21900.012.53489340
AGCTTAT14800.012.4695591
ATGCGCC9500.012.31509210
ATGGCCC9900.012.04607740
Produced by FastQC (version 0.11.2)