FastQCFastQC Report
Fri 13 Feb 2015
MRP30_CAGATC_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP30_CAGATC_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4902132
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAC7100.018.38018621
AGCTTTG23050.017.1783771
TGCCCGG7700.016.65575619
GCCCGGA7600.016.2828120
CCCGAGC8100.015.8317981
AGCTTAC10100.015.5925461
CCGGACA8950.015.08279722
CGGACAG9250.013.86351923
AGCTTAG7300.013.5603281
AGCTTAT16600.013.1463151
AGCTTTC21700.012.9595731
TTGCCCG10250.012.95088818
CCGAGCG10700.012.8257272
AGCTTCC19800.012.6124121
AGCTTTT41000.012.4012351
CGAGCGT11500.012.3247643
AGCTTGT17200.011.9029151
AGCTTTA23000.011.7379911
AGCTTGA19250.011.5703061
GAGCGTG11150.011.5007744
Produced by FastQC (version 0.11.2)