FastQCFastQC Report
Fri 13 Feb 2015
MRP30_CAGATC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP30_CAGATC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4902132
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAC10300.017.69425221
GCCCGGA10800.017.29103520
CCCGAGC11100.017.2340221
TGCCCGG11400.016.18328919
CCGGACA12150.015.1852522
CGGACAG12300.015.18299223
AGCTTGG11800.015.0673131
CCGAGCG12700.014.7050872
TTGCCCG13500.013.83254618
GCGTGAA14650.013.207716
AGCTTTG22750.013.15712451
AGCTTAC11200.013.061291
GAGCGTG14500.012.878714
AGCTTCC20850.012.4131641
CGAGCGT16200.012.2217773
AGCTTTA21800.012.1819341
AGCTTAT17800.012.0114181
AGCTTGC14050.011.8534871
GGACAGT15650.011.78990924
GAGCAGT16800.011.78588641
Produced by FastQC (version 0.11.2)