FastQCFastQC Report
Fri 13 Feb 2015
MRP29_GCCAAT_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP29_GCCAAT_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3304895
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAC5800.018.23340821
GCCCGGA6450.016.74478320
AGCTTAC7700.016.3627341
AGCTTTG16750.015.581231
TGCCCGG7100.014.894896519
CGGACAG7300.014.177323
CCGAGCG7300.014.1768712
CCCGAGC7350.014.0808571
CGCCTTA7800.013.8460213
AGCTTTT28700.013.6403631
ATGGCCC7800.013.55797140
CCGGACA8200.013.44519422
ATGCGCC8100.013.33300210
TGCGCCT8500.013.23496411
AGCTTAT11850.013.1005441
AGCTTTA15350.013.0448831
TTGCCCG8300.013.01251218
AGCTTCC14000.012.8564331
AATGCGC8400.012.8562399
AGCTTGC9600.012.421191
Produced by FastQC (version 0.11.2)