FastQCFastQC Report
Fri 13 Feb 2015
MRP29_GCCAAT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP29_GCCAAT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3304895
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAC7650.021.76411221
GCCCGGA8100.020.55437520
CGGACAG8350.019.67012223
CCCGAGC8700.018.8853681
TGCCCGG8650.018.72724719
CCGAGCG9250.017.7576182
TTGCCCG9750.017.07594118
GAGCGTG10100.016.4849474
CGAGCGT10250.016.2437063
CCGGACA10150.016.18182422
GCGTGAA10800.015.4164816
GGACAGT11000.014.5238724
AGCTTAC7150.013.8505971
AGCTTTG14350.013.8023371
GAGCAGT12350.013.4822341
ATGGAGC12600.013.39330338
AGCTTTC13300.013.0304951
AGCTTTA15350.012.4632811
AGCTTCC14500.012.4177771
AGCGTGA14000.012.0534255
Produced by FastQC (version 0.11.2)