FastQCFastQC Report
Fri 13 Feb 2015
MRP28_ACAGTG_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP28_ACAGTG_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5694539
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAGC11100.024.727081
CCCGGAC11600.024.0514721
CCGAGCG11950.022.5913242
TGCCCGG12750.022.23486519
GCCCGGA13050.021.55130820
CGGACAG14000.019.92678623
CCGGACA14500.019.55066122
CGAGCGT14450.018.9943373
GAGCGTG14650.018.7345374
TTGCCCG15350.018.61527618
CGCCTTA11600.017.06788413
ATGCGCC11900.016.82696210
GCGTGAA16950.016.7221096
GGACAGT17400.016.03501924
TGCGCCT12000.015.93677311
GCGCCTT12300.015.5480712
ATGGCCC12900.014.65293940
GAGCAGT19700.014.39336641
AATGCGC13550.014.2792899
AGCGTGA20400.014.2250435
Produced by FastQC (version 0.11.2)