FastQCFastQC Report
Fri 13 Feb 2015
MRP28_ACAGTG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP28_ACAGTG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5694539
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGCGTGAAAGGATTTGCCCGGACAGTTTGCTTCATGGAGCAGTTTTT66120.11611124271868188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAGC13050.021.5582471
CCGAGCG12900.021.4535712
CCCGGAC13300.021.3147521
GCCCGGA13700.020.52801720
TGCCCGG13950.020.1601319
CGGACAG14950.019.1127823
CGAGCGT15300.018.3816183
TTGCCCG15900.017.97066318
CCGGACA15700.017.9131422
GAGCGTG16200.017.3604164
ATGCGCC13250.016.97985610
ATGGCCC14700.015.46005840
TGCGCCT14900.015.40206811
GCGTGAA18800.015.0791856
CGCCTTA15100.015.04906713
AATGCGC15600.014.8546529
GGACAGT19400.014.8458124
GCGCCTT15850.014.62086612
GGCCCAA16750.013.9700842
GAGCAGT20150.013.84645141
Produced by FastQC (version 0.11.2)