FastQCFastQC Report
Fri 13 Feb 2015
MRP26_CCGTCC_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP26_CCGTCC_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20556556
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGGG32200.027.1101553
GGGACGG31900.026.0952112
CGGGACG18650.024.6098861
CGGGTGA26800.021.8242196
ACGGGTG28500.020.2859735
GGGTGAC31350.020.0201267
AGCTTCC71200.020.0023961
GACGGGT30550.018.557894
AGCTTAG28000.017.4365271
AGCTTGC47500.016.5306551
AGCTTCG45850.016.4876291
AGCTTCA130150.016.3878671
AGCTTAC38850.016.3311331
ACGGGGG17150.016.0030695
AGCTTCT126150.015.98008251
GGTCGCC22100.015.171943
AGCTTGG47500.014.8728521
GACGGGG17800.014.7878534
CGGGGGA21450.014.7874046
AGCTTTG71150.014.6407841
Produced by FastQC (version 0.11.2)