FastQCFastQC Report
Fri 13 Feb 2015
MRP26_CCGTCC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP26_CCGTCC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20556556
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG16450.022.84212735
AGCTTCC74600.019.0061151
GTCCCGA22300.017.35528236
CCGTCCC22350.017.21548734
CCCGTCC23000.016.72859633
CACCCGT24200.016.5531
AGCTTCG44950.016.5225031
AGCTTAG26200.015.4618991
ACCCGTC25050.015.3595932
AGCTTAC37600.015.3229961
GTCACCC26750.015.30983529
AGCTTCA135950.015.2300111
TCACCCG26300.015.22947930
AGCTTGC50300.014.451941
AGCTTTG74250.014.3975561
AGCTTGG44450.014.3793151
AGCTTAT57250.014.1913381
AGCTTCT128800.013.786461
AGCTTTC79700.012.848271
AGCTTAA73050.012.6931261
Produced by FastQC (version 0.11.2)