FastQCFastQC Report
Fri 13 Feb 2015
MRP25_ATGTCA_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP25_ATGTCA_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23901562
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTCC101500.013.7871131
AGCTTAC52200.011.7663331
AGCTTTG101900.011.7017461
GGTCGCC16600.011.38721343
CCCGAGC25200.010.1765946
CCGAGCG24600.010.059027
AGCTTCA110300.010.0558871
AGCTTTC94750.010.0203351
AGCTTAT77850.09.9992281
AGCTTCT120450.09.7501811
CCCGGAC27500.09.65452726
GCCCGGA28600.09.44093625
TCCCGAG27800.09.3868815
AGCTTTA100550.09.2857071
TGCCCGG28450.09.25336724
AGCTTGG58900.09.2437681
AGCTTAG29700.09.0144561
GCTTCCC50650.08.9280292
AGCTTAA66100.08.8155071
AGCTTGT73900.08.7679291
Produced by FastQC (version 0.11.2)