FastQCFastQC Report
Fri 13 Feb 2015
MRP25_ATGTCA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP25_ATGTCA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23901562
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTCC103850.013.9569371
AGCTTAC52050.011.1560321
CCGAGCG30000.011.024317
CCCGAGC30850.010.7210776
AGCTTTG99700.010.4519331
TCCCGAG32550.010.2302685
CCCGGAC32550.09.95402326
GCCCGGA33900.09.82311425
GCTTCCC57100.09.73323252
TGCCCGG34550.09.70347324
CTTCCCG42050.09.4172643
AGCTTGG57700.09.2835041
AGCTTCA113100.09.1737961
AGCTTTC88600.09.1703141
AGCTTGC58150.09.1342531
CGGACAG37900.09.08368728
AGCTTAG30000.09.0026581
CGAGCGT37450.08.9513768
AGCTTCT122550.08.8887931
GCACACG25600.08.52494611
Produced by FastQC (version 0.11.2)