FastQCFastQC Report
Fri 13 Feb 2015
MRP23_GATCAG_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP23_GATCAG_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20424270
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAGC22500.016.6987781
CCGAGCG23700.015.7579542
CCCGGAC24500.015.33662721
TGCCCGG25600.014.85341119
GCCCGGA26250.014.14275720
CGGACAG29300.013.20741923
GGTCGCC13750.012.43813443
CGAGCGT30650.012.03809453
CCGGACA31900.011.91959922
TTGCCCG34950.011.00842518
GAGCGTG35050.010.7192214
GCGTGAA40000.09.7858496
AGCTTTG74300.09.6291741
AGCGTGA41350.09.3031695
TGGTCGC21700.08.9183242
GTGGTCG21600.08.85527541
GTCGCCG20000.08.77629344
GGACAGT45650.08.67497724
CTCGGTG22650.08.14609737
GCGTCGT22250.07.98853111
Produced by FastQC (version 0.11.2)