FastQCFastQC Report
Fri 13 Feb 2015
MRP23_GATCAG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP23_GATCAG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20424270
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAC28750.016.51251621
CCGAGCG28700.016.4637362
CCCGAGC29800.016.0106281
GCCCGGA30750.015.51147320
TGCCCGG31550.015.40336419
CGGACAG34800.013.90048623
CGAGCGT36100.013.4622723
CCGGACA36650.013.19882422
GAGCGTG40250.012.018254
TTGCCCG41500.011.92709618
GCGTGAA45000.010.9996496
GCACACG21100.010.236464511
AGCGTGA49850.09.7038025
GGACAGT51450.09.53399424
AGTCACG22200.09.22382928
GAGCAGT55800.08.95194141
CGATCAG24800.08.70982633
ATGGAGC60800.08.43795338
GTCACGA25100.08.42705729
GCGCACT30500.08.2618915
Produced by FastQC (version 0.11.2)