FastQCFastQC Report
Fri 13 Feb 2015
MRP22_TGACCA_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP22_TGACCA_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8394616
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAC23100.020.8432751
AGCTTTG49500.020.5445861
AGCTTAT34650.017.9862521
AGCTTTC38950.016.9825951
AGCTTCC37550.016.9566751
AGCTTAG12950.016.8525711
AGCTTTT81650.016.7537331
AGCTTTA47150.016.1286951
AGCTTGT30800.015.413311
AGCTTGG24800.015.1505711
AGCTTCT50350.014.8802051
AGCTTAA26750.014.8031131
AGCTTCA42900.014.3175761
AGCTTCG26700.014.0724381
AGCTTGC27200.012.9038671
AGCTTGA31800.012.0985461
TCCCGAG12600.011.2485875
GCTTCCC19250.010.9863532
CCCGGAC13400.010.24259326
GCTTTGT32300.010.0303572
Produced by FastQC (version 0.11.2)