FastQCFastQC Report
Fri 13 Feb 2015
MRP22_TGACCA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP22_TGACCA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8394616
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTTG48250.019.7301851
AGCTTAC21550.018.3803371
AGCTTCC39900.017.3162381
AGCTTAG11700.016.3501261
AGCTTGG22850.016.2512151
AGCTTTA40350.016.1749341
AGCTTAT35250.015.5144091
AGCTTTT73150.015.1369661
AGCTTTC37750.014.7850491
AGCTTGT31750.014.105791
GCTTCCC21400.013.5635532
AGCTTCT47550.013.5364121
AGCTTGC26200.013.4861011
AGCTTAA23500.012.9286741
AGCTTCA40600.012.638541
TCCCGAG14550.012.5255355
AGCTTCG28800.011.9560321
AGCTTGA31200.011.3248681
CCCGGAC16450.011.07954226
CGGACAG17900.010.30798728
Produced by FastQC (version 0.11.2)