FastQCFastQC Report
Fri 13 Feb 2015
MRP20_CGATGT_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP20_CGATGT_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10788163
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTTG68850.020.65221
AGCTTAC31650.019.1930181
AGCTTAT46800.017.9795571
AGCTTCC53600.017.4195331
AGCTTAG18750.017.2788331
AGCTTTT114750.017.234131
AGCTTTA62600.016.8918321
AGCTTTC52450.016.7290921
AGCTTGG33850.015.752261
AGCTTCT70300.015.7137331
AGCTTGC30550.015.4654051
AGCTTAA34000.015.2195621
AGCTTCG30850.014.294011
AGCTTGT45400.014.0739391
AGCTTGA47300.014.0318221
AGCTTCA59200.013.60551
CCCGAGC14900.013.1351766
CCCGGAC14500.012.87969526
GCTTCCC25500.012.704732
TCCCGAG16550.011.9618855
Produced by FastQC (version 0.11.2)