FastQCFastQC Report
Fri 13 Feb 2015
MRP20_CGATGT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP20_CGATGT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10788163
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTTG64050.018.2027381
AGCTTAC31200.017.3134161
AGCTTTA58550.016.8373361
AGCTTTC50650.016.4417651
AGCTTCC51500.016.3889141
AGCTTGG33700.016.0958251
AGCTTGC31700.016.0463141
AGCTTAG16750.015.9903611
AGCTTTT104800.015.9570881
AGCTTAT47200.015.4023381
TCCCGAG17950.014.1646965
AGCTTGT45000.014.154681
CCCGGAC17000.013.49989626
AGCTTCT67800.013.4115131
AGCTTCG34050.013.2863311
AGCTTCA58500.013.1197221
AGCTTAA35300.012.9433731
CCCGAGC19050.012.8743346
AGCTTGA45100.012.7758431
GCTTCCC26700.012.7256242
Produced by FastQC (version 0.11.2)