FastQCFastQC Report
Fri 13 Feb 2015
MRP19_ATCACG_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP19_ATCACG_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10514927
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTTG65450.019.3872361
AGCTTAC30900.019.0760861
AGCTTAT47950.017.0788731
AGCTTGG32150.016.6549151
AGCTTTC51700.016.2752441
AGCTTTA60400.015.68164351
AGCTTGC32000.015.3268531
AGCTTCC48100.015.2949871
AGCTTAG17650.014.9137751
AGCTTTT99700.014.8708811
AGCTTCT68150.014.8226861
AGCTTGT42400.014.4327631
AGCTTGA44500.013.5999941
AGCTTCA57000.013.5383491
AGCTTCG31750.013.1091481
CCCGGAC13400.012.59319626
AGCTTAA33900.012.4768391
TCCCGAG13950.012.2558815
CCCGAGC14750.011.5908796
GCCCGGA15200.011.25045725
Produced by FastQC (version 0.11.2)