FastQCFastQC Report
Fri 13 Feb 2015
MRP19_ATCACG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP19_ATCACG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10514927
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTTG60900.016.890451
AGCTTCC50200.016.0068861
AGCTTTC49250.015.5844141
AGCTTTA57600.015.4353651
AGCTTAC29450.015.2857591
AGCTTAT44250.014.7003231
AGCTTGG31500.014.0766071
AGCTTTT98000.013.8954181
AGCTTCT63400.013.3132571
AGCTTAG15900.013.165221
TCCCGAG16000.013.0783345
AGCTTAA35500.012.8709321
AGCTTGT39400.012.453821
CCCGAGC17000.012.4413756
AGCTTGA43800.012.4360821
AGCTTGC33400.012.1976011
CCCGGAC17550.012.17968126
GCTTCCC26650.012.158662
AGCTTCG33300.012.0990461
AGCTTCA59350.012.0221141
Produced by FastQC (version 0.11.2)