FastQCFastQC Report
Fri 13 Feb 2015
MRP18_AGTTCC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP18_AGTTCC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12636188
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC15600.028.41512743
CCGAGCG21050.026.935632
CCCGAGC21850.026.0524041
GTCGCCG18600.024.55798744
CCCGGAC24300.023.42581421
GCCCGGA25300.022.4109620
CTCGGTG21200.021.75688437
GTGGTCG20900.021.74768641
TGGTCGC21000.021.64412542
TGCCCGG26850.021.36861419
ATCTCGG21800.021.36449235
AGCGTCG21150.021.06364610
CGGACAG27250.021.05494523
GTCGTGT20950.020.72774313
GTGTAGG22050.020.10186416
CGTGTAG21750.020.06879215
CGAGCGT28300.020.035163
GCGTCGT22050.019.99982511
CCGGACA29650.019.4265522
GAGCGTG30850.018.816694
Produced by FastQC (version 0.11.2)