FastQCFastQC Report
Fri 13 Feb 2015
MRP18_AGTTCC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP18_AGTTCC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12636188
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGCGTGAAAGGATTTGCCCGGACAGTTTGCTTCATGGAGCAGTTTTT148860.1178045150958501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCG26300.027.380322
CCCGAGC27050.026.7139741
CCCGGAC31600.022.78444521
GCCCGGA31950.022.53485120
GCACACG22550.021.85100411
TGCCCGG32850.021.84896519
ACACGTC22900.021.12403313
CGGACAG34550.020.90415223
CGAGCGT34750.020.7190113
CACACGT23800.020.51429712
CCGGACA36600.019.67181622
GAGCGTG36600.019.6102624
CACGTCT25250.019.06892614
TTGCCCG38900.018.7400618
AGTTCCG25750.018.61127934
GCGTGAA39650.018.385516
CCGTATC23850.018.11291738
CGTCTGA27450.017.95046216
ACGTCTG27800.017.64353215
AGCACAC29350.016.86508210
Produced by FastQC (version 0.11.2)