FastQCFastQC Report
Fri 13 Feb 2015
MRP17_AGTCAA_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP17_AGTCAA_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12913809
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAC42650.018.6217331
AGCTTTG79650.018.4455591
AGCTTAT55550.017.9830471
CCGAGCG20850.017.804462
CCCGAGC21150.017.5524581
CCCGGAC21800.017.02950321
GCCCGGA22150.016.86218620
AGCTTTT124350.016.5192151
TGCCCGG23750.015.91568119
AGCTTGT53000.015.7068911
AGCTTCC58800.015.6881211
AGCTTTC67450.015.344061
CGGACAG24700.015.30247123
AGCTTTA78800.014.8185431
CGAGCGT25100.014.7898763
AGCTTGC36900.014.7554821
AGCTTGG41600.014.6027551
AGCTTAA42250.014.5911061
CCGGACA27050.013.97337722
AGCTTAG22650.013.9067051
Produced by FastQC (version 0.11.2)