FastQCFastQC Report
Fri 13 Feb 2015
MRP17_AGTCAA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP17_AGTCAA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12913809
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAC38800.017.9817731
CCCGAGC24500.017.5456661
CCCGGAC25050.017.15551621
CCGAGCG25150.016.9983652
GCCCGGA25800.016.56940720
AGCTTAT51950.015.6395481
TGCCCGG27800.015.62004319
AGCTTTG73900.015.5320341
CGGACAG28350.015.07921323
AGCTTTA70300.014.6626591
AGCTTAA38650.014.6159421
CGAGCGT30650.014.4614623
AGCTTGC41350.014.3691471
AGCTTCC61950.014.3138831
AGCTTGG38850.014.1931121
CCGGACA31750.013.67702722
AGCTTAG21150.013.6207851
AGCTTTT116900.013.5152841
AGCTTTC62100.013.4819871
GAGCGTG33500.013.0967814
Produced by FastQC (version 0.11.2)