FastQCFastQC Report
Fri 13 Feb 2015
MRP16_CTTGTA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP16_CTTGTA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13285113
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGCGTGAAAGGATTTGCCCGGACAGTTTGCTTCATGGAGCAGTTTTT164280.12365720938918622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC17700.029.49243243
CCCGAGC29450.028.5736561
CCGAGCG30500.027.4424322
GTCGCCG21250.024.56555444
CCCGGAC34450.024.23096521
CTCGGTG22500.023.99983237
GCCCGGA35600.023.51142720
ATCTCGG23650.023.02309635
GTCGTGT23700.022.97452413
TGCCCGG36600.022.74608619
CGAGCGT37300.022.37923
TGGTCGC23450.022.2608142
GCGTCGT24250.022.08232111
GTGGTCG24550.021.72164541
CGGACAG38800.021.6303323
TCGCCGT22650.021.35835345
GAGCGTG40250.021.0743894
CCGGACA40700.020.62056222
AGCGTCG26450.020.33067110
TTGCCCG41750.020.10165618
Produced by FastQC (version 0.11.2)