FastQCFastQC Report
Fri 13 Feb 2015
MRP16_CTTGTA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP16_CTTGTA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13285113
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGCGTGAAAGGATTTGCCCGGACAGTTTGCTTCATGGAGCAGTTTTT221180.16648710477660222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAGC34200.028.2368971
CCGAGCG34000.028.1277872
GCACACG22750.026.10952811
CCCGGAC39600.024.09066621
GCCCGGA41050.023.2944420
TGCCCGG42650.022.31513219
CGGACAG43500.021.77564223
CACACGT27700.021.7686512
ACACGTC27600.021.6030413
CGAGCGT44800.021.394713
GAGCGTG45250.021.2813914
CACGTCT28400.021.2321814
CCGGACA46100.020.69393322
ACGTCTG29750.020.11744315
TTGCCCG49100.019.6586618
GCGTGAA49400.019.4480386
CGTCTGA31400.019.34693716
GTATGCC32600.018.49730345
CAGTCAC33900.018.3847727
GAACTCC33950.018.092621
Produced by FastQC (version 0.11.2)