FastQCFastQC Report
Fri 13 Feb 2015
MRP15_GGCTAC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP15_GGCTAC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7143029
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAGC8750.021.3427771
CCGAGCG8700.021.2068182
CCCGGAC10450.017.87087221
AGCTTTG27850.017.4505631
TGCCCGG10700.017.24304819
GCCCGGA11100.016.62167520
AGCCGTG5850.015.7691723
CGAGCGT11850.015.7594363
AGCTTTA31100.015.409911
CGGACAG12550.014.88052723
AGCTTTC29000.014.8189111
AGCTTGC18300.014.7540441
GCCGTGA5500.014.3181284
AGCTTAC12900.014.3022731
AGCTTGA25050.014.1017441
AGCTTCC23550.014.0445341
CCGGACA13300.014.04139922
TTGCCCG14400.013.749948518
AGCTTGT20950.013.7469651
GAGCGTG13950.013.7096264
Produced by FastQC (version 0.11.2)