FastQCFastQC Report
Fri 13 Feb 2015
MRP15_GGCTAC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP15_GGCTAC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7143029
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAGC8650.022.6412261
CCGAGCG9250.021.1646022
AGCTTTG32100.017.3917561
CCCGGAC12250.015.97942121
AGCTTAC13300.015.4023341
GCCCGGA12850.015.23319220
TGCCCGG13450.015.05560119
AGCTTTA30550.015.0319651
AGCTTGC16950.014.4761681
CGAGCGT13750.014.3997453
AGCTTGA26050.014.2584731
AGCTTTC27600.014.1102211
AGCTTCA33900.014.0113361
CGGACAG14750.013.57612523
AGCTTGG16000.013.5066631
CCGGACA15200.013.026175522
AGCTTAA17000.012.5797351
AGCTTCG17250.012.5279191
AGCTTCC26750.012.3705881
AGCTTAG9000.012.2560451
Produced by FastQC (version 0.11.2)