##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n02 9-9610.34200000001ba0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11329192 Sequences flagged as poor quality 0 Sequence length 101 %GC 30 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.047945519857024 38.0 38.0 38.0 32.0 38.0 2 35.857925348956925 38.0 38.0 38.0 32.0 38.0 3 36.09235318811792 38.0 38.0 38.0 32.0 38.0 4 36.0004664939918 38.0 38.0 38.0 32.0 38.0 5 35.91527003867531 38.0 38.0 38.0 32.0 38.0 6 38.168323831037554 40.0 38.0 40.0 38.0 40.0 7 38.182837134369336 40.0 38.0 40.0 38.0 40.0 8 38.260003802565976 40.0 38.0 40.0 38.0 40.0 9 38.250963793357904 40.0 38.0 40.0 38.0 40.0 10-11 38.272221090436105 40.0 38.0 40.0 38.0 40.0 12-13 38.229351131130976 40.0 38.0 40.0 38.0 40.0 14-15 38.19423604966709 40.0 38.0 40.0 38.0 40.0 16-17 38.18423599847191 40.0 38.0 40.0 38.0 40.0 18-19 38.162730228245756 40.0 38.0 40.0 38.0 40.0 20-21 38.15144923839229 40.0 38.0 40.0 38.0 40.0 22-23 38.13230484574716 40.0 38.0 40.0 38.0 40.0 24-25 38.07933513705126 40.0 38.0 40.0 38.0 40.0 26-27 38.06420563796606 40.0 38.0 40.0 38.0 40.0 28-29 38.00089048715919 40.0 38.0 40.0 38.0 40.0 30-31 37.9697357499105 40.0 38.0 40.0 38.0 40.0 32-33 37.97512973564223 40.0 38.0 40.0 38.0 40.0 34-35 37.89988818267004 40.0 38.0 40.0 38.0 40.0 36-37 37.91880458023838 40.0 38.0 40.0 38.0 40.0 38-39 37.877000407443006 40.0 38.0 40.0 38.0 40.0 40-41 37.82610631014109 40.0 38.0 40.0 35.0 40.0 42-43 37.683434529135 40.0 38.0 40.0 32.0 40.0 44-45 37.617588129850745 40.0 38.0 40.0 32.0 40.0 46-47 37.5529256631894 40.0 38.0 40.0 32.0 40.0 48-49 37.48754394841221 40.0 38.0 40.0 32.0 40.0 50-51 37.43607937794681 40.0 38.0 40.0 32.0 40.0 52-53 37.371646274509246 40.0 38.0 40.0 32.0 40.0 54-55 37.328098641103445 40.0 38.0 40.0 32.0 40.0 56-57 37.312273858541715 40.0 38.0 40.0 32.0 40.0 58-59 37.25076889861165 40.0 38.0 40.0 32.0 40.0 60-61 37.22084341937183 40.0 38.0 40.0 32.0 40.0 62-63 37.15419157871099 40.0 38.0 40.0 32.0 40.0 64-65 37.147679419679704 38.0 38.0 40.0 32.0 40.0 66-67 37.086805705120014 38.0 38.0 40.0 32.0 40.0 68-69 37.04298051440915 38.0 38.0 40.0 32.0 40.0 70-71 36.94444431694687 38.0 38.0 40.0 32.0 40.0 72-73 36.88384648260882 38.0 38.0 40.0 32.0 40.0 74-75 36.809955643791724 38.0 38.0 40.0 32.0 40.0 76-77 36.738490529598224 38.0 38.0 40.0 32.0 40.0 78-79 36.66402171487604 38.0 38.0 40.0 32.0 40.0 80-81 36.60067747991207 38.0 38.0 40.0 32.0 40.0 82-83 36.524773478991264 38.0 38.0 40.0 32.0 40.0 84-85 36.43561297222256 38.0 38.0 40.0 32.0 40.0 86-87 36.38227942469331 38.0 38.0 40.0 32.0 40.0 88-89 36.32053962895147 38.0 38.0 40.0 27.0 40.0 90-91 36.219114743575716 38.0 38.0 40.0 27.0 40.0 92-93 36.13089393312427 38.0 38.0 40.0 27.0 40.0 94-95 36.04268724548052 38.0 38.0 40.0 27.0 40.0 96-97 35.97764205073054 38.0 38.0 40.0 27.0 40.0 98-99 35.857050617555075 38.0 38.0 40.0 27.0 40.0 100-101 34.080934853959576 38.0 35.0 39.0 20.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.1412653867852569 2201 2 0.27117789512119117 2201 3 0.2669273284970828 2201 4 0.15274522953525604 2201 5 0.23761615095336452 2201 6 0.22694006018361534 2201 7 0.25247276244587624 2201 8 0.13533432166735793 2201 9 0.16296390160111685 2201 10-11 0.1542679137577494 2201 12-13 0.15904680771978974 2201 14-15 0.12650279647370155 2201 16-17 0.15666425393611405 2201 18-19 0.15025819569125076 2201 20-21 0.17733242466635346 2201 22-23 0.12966448553289922 2201 24-25 0.14886033508956587 2201 26-27 0.1740163216666275 2201 28-29 0.1767556818143987 2201 30-31 0.12431853945609816 2201 32-33 0.12879597928208142 2201 34-35 0.14614971162320956 2201 36-37 0.052046446692010306 2201 38-39 0.09562049173603526 2201 40-41 0.10209855213213359 2201 42-43 0.14524282190937754 2201 44-45 0.13210023403337345 2201 46-47 0.12906007314563794 2201 48-49 0.003912318881546639 2201 50-51 0.10684177781655535 2201 52-53 0.08895216687745489 2201 54-55 0.13714293998442173 2201 56-57 0.01141446546450453 2201 58-59 0.07218811733052632 2201 60-61 0.03543023644353127 2201 62-63 0.1226387485636522 2201 64-65 0.07617712303517266 2201 66-67 0.02455636887223278 2201 68-69 0.024519432038090372 2201 70-71 0.11178013340611415 2201 72-73 -0.010419730869898558 2201 74-75 -0.008027052897610076 2201 76-77 0.03194107781359179 2201 78-79 0.07083050964886439 2201 80-81 0.11774682125057723 2201 82-83 0.05682416409155877 2201 84-85 -0.013033808073139141 2201 86-87 0.05025188721284479 2201 88-89 0.0617493521591328 2201 90-91 -0.01210895346309826 2201 92-93 -0.05013861946228104 2201 94-95 0.0817159315399607 2201 96-97 -0.11915702774776094 2201 98-99 -0.07568915382238117 2201 100-101 -0.03585605424726168 1101 1 0.15323599517162734 1101 2 0.11735221743503388 1101 3 0.20204617193477503 1101 4 0.11827451757157803 1101 5 0.17205940583975377 1101 6 0.20556432686313997 1101 7 0.23360027585633958 1101 8 0.1771011147981696 1101 9 0.2238955604877546 1101 10-11 0.21197295490392776 1101 12-13 0.21726193147192419 1101 14-15 0.20887324308721844 1101 16-17 0.15998629066056225 1101 18-19 0.1768521172019959 1101 20-21 0.2671380955768896 1101 22-23 0.23082865921674767 1101 24-25 0.172453885139646 1101 26-27 0.1886664377202294 1101 28-29 0.19905413915584091 1101 30-31 0.24697545186714365 1101 32-33 0.1957997420649562 1101 34-35 0.21351937573759017 1101 36-37 0.21092146870754647 1101 38-39 0.16888277903061066 1101 40-41 0.22324491065874952 1101 42-43 0.1909901940143186 1101 44-45 0.16474341521877278 1101 46-47 0.1642252197959735 1101 48-49 0.13829214595973127 1101 50-51 0.17294300621905734 1101 52-53 0.14454585018471988 1101 54-55 0.17790224781293063 1101 56-57 0.11036285852407701 1101 58-59 0.09084620596651405 1101 60-61 0.04770703430334322 1101 62-63 0.09651847981980666 1101 64-65 0.11516838493742654 1101 66-67 0.07646644113692247 1101 68-69 0.06591712490513402 1101 70-71 0.11934483686094666 1101 72-73 0.08274841858873572 1101 74-75 0.10361387644794462 1101 76-77 0.14059364385553152 1101 78-79 0.09570450125148255 1101 80-81 0.16811733844908616 1101 82-83 0.15964125225311676 1101 84-85 0.12562373037428642 1101 86-87 0.21100453653681228 1101 88-89 0.14447007374855758 1101 90-91 0.11146494759144332 1101 92-93 0.20837270271720598 1101 94-95 0.13294789733924972 1101 96-97 0.08537404739604426 1101 98-99 0.04235814120288239 1101 100-101 0.2313623006025125 2202 1 0.03806046351052572 2202 2 0.08702717765289947 2202 3 0.22852392739715555 2202 4 0.06186719898668969 2202 5 0.023317007177844573 2202 6 0.11608841298950523 2202 7 0.16277769200643633 2202 8 0.09487055176615655 2202 9 0.15551169614239058 2202 10-11 0.12682353778340882 2202 12-13 0.05943441727855259 2202 14-15 0.09619536760040859 2202 16-17 0.07635201197606278 2202 18-19 0.11109474246779882 2202 20-21 0.10457526377668813 2202 22-23 0.11782109689350762 2202 24-25 0.08824463880205258 2202 26-27 0.09234808759646995 2202 28-29 0.0882896474656576 2202 30-31 0.11096943307558149 2202 32-33 0.13061595175793883 2202 34-35 0.13754913126165036 2202 36-37 0.1409346771880422 2202 38-39 0.14555011718785948 2202 40-41 0.10890927377369763 2202 42-43 0.08844487120022393 2202 44-45 0.008726800151976022 2202 46-47 -0.0387369605268546 2202 48-49 0.005401586015977955 2202 50-51 0.06897259410063583 2202 52-53 0.09694820299307594 2202 54-55 0.08025212777749147 2202 56-57 0.030105718040879026 2202 58-59 0.07318614460768913 2202 60-61 -0.02141721352145254 2202 62-63 0.006337826961548387 2202 64-65 -0.006440240317793666 2202 66-67 -0.010032136306314499 2202 68-69 0.06609280584217458 2202 70-71 0.012123553700426726 2202 72-73 -0.03991766825961918 2202 74-75 -0.010609120864607746 2202 76-77 -0.06031657142710145 2202 78-79 -0.061388052157873574 2202 80-81 -0.03640250944608425 2202 82-83 -0.044354139350943456 2202 84-85 0.004980785736691473 2202 86-87 -0.0047548248696571704 2202 88-89 -0.056289291189081325 2202 90-91 0.03998440478264342 2202 92-93 -9.709397621477933E-4 2202 94-95 0.016082657384117738 2202 96-97 -0.08101711740428641 2202 98-99 -0.05438120891007969 2202 100-101 0.007750419651834761 1102 1 0.01567336989114665 1102 2 0.016934919641094837 1102 3 0.07459622291668211 1102 4 -0.05062003301735274 1102 5 0.0031878165712484474 1102 6 -0.02458201975517227 1102 7 0.09354525920021217 1102 8 0.07922961454586641 1102 9 0.08475410682023465 1102 10-11 0.03709146873782743 1102 12-13 0.135503080381433 1102 14-15 0.11240811704936249 1102 16-17 0.13876904018204783 1102 18-19 0.08042508608211563 1102 20-21 0.1573484397920808 1102 22-23 0.17124076171215563 1102 24-25 0.16379947256699268 1102 26-27 0.11818047798364262 1102 28-29 -0.011215225245990723 1102 30-31 0.07191692814690498 1102 32-33 0.0788478375440107 1102 34-35 0.10817751425304323 1102 36-37 0.11141395216829864 1102 38-39 0.09410462680072129 1102 40-41 0.08092494849580589 1102 42-43 -0.010639234105752848 1102 44-45 -0.003368236506830158 1102 46-47 0.005310458492907344 1102 48-49 -0.03376192451234772 1102 50-51 -0.021302332167671523 1102 52-53 0.00653047163384457 1102 54-55 -0.01782526601385115 1102 56-57 -0.07681073292670959 1102 58-59 -0.04043698227386017 1102 60-61 -0.027241542442759226 1102 62-63 -0.09617678290492648 1102 64-65 -0.1653522229875577 1102 66-67 -0.17499791041160506 1102 68-69 -0.1931241692620418 1102 70-71 -0.05531923624537427 1102 72-73 -0.07312711439092823 1102 74-75 -0.020224201350750093 1102 76-77 -0.026246354873030953 1102 78-79 -0.04388609272043453 1102 80-81 -0.05651490036311202 1102 82-83 -0.099933901496577 1102 84-85 -0.09524766177328559 1102 86-87 -0.10884395676554703 1102 88-89 -0.12397046358417896 1102 90-91 -0.24785935839587125 1102 92-93 -0.20794033481779906 1102 94-95 -0.13244245729931947 1102 96-97 -0.1520911579316504 1102 98-99 -0.19145342950567823 1102 100-101 -0.1471935302278382 2203 1 -0.01681223549206834 2203 2 -0.0854711074874217 2203 3 0.06204050528778993 2203 4 -0.04659148902261023 2203 5 -0.03901763821149018 2203 6 -0.040533175769219554 2203 7 0.08291989463647553 2203 8 0.10723122929203299 2203 9 0.09206245961176762 2203 10-11 0.1015655869040657 2203 12-13 0.12636870488903185 2203 14-15 0.08486853585014131 2203 16-17 0.11585789826281001 2203 18-19 0.06167926392929246 2203 20-21 0.08713590810965144 2203 22-23 0.037215094925642234 2203 24-25 0.04424700647729196 2203 26-27 0.008742373142254678 2203 28-29 -0.008908869167839839 2203 30-31 -0.019749328696647694 2203 32-33 -0.028675253510691334 2203 34-35 -0.015963757644506416 2203 36-37 -0.06006863815787966 2203 38-39 -0.027943588807019637 2203 40-41 0.020351307832541465 2203 42-43 -0.012556355184798917 2203 44-45 -0.0638864777897652 2203 46-47 -0.15686312472099218 2203 48-49 -0.11177054589789748 2203 50-51 -0.04325702622648464 2203 52-53 -0.0902414852709299 2203 54-55 -0.05190979563852949 2203 56-57 -0.1998409273838604 2203 58-59 -0.08228822663559754 2203 60-61 -0.1713132386138554 2203 62-63 -0.1487952462197697 2203 64-65 -0.18469250843785545 2203 66-67 -0.19380011448600243 2203 68-69 -0.14486724522938488 2203 70-71 -0.11172603161769956 2203 72-73 -0.15550983908909188 2203 74-75 -0.20208852480138972 2203 76-77 -0.16789457648288675 2203 78-79 -0.24016773052633056 2203 80-81 -0.18664515102678791 2203 82-83 -0.2064133269195665 2203 84-85 -0.23631240256951003 2203 86-87 -0.37346624947446827 2203 88-89 -0.32350160439228404 2203 90-91 -0.2317552728567307 2203 92-93 -0.30266334420780794 2203 94-95 -0.2459466409340081 2203 96-97 -0.2837369030205039 2203 98-99 -0.29722854309132885 2203 100-101 -0.11269720163102193 1103 1 -0.03940563097528127 1103 2 -0.07424457912398452 1103 3 -0.03566786498970487 1103 4 -0.026117968894389776 1103 5 -0.011819415088133667 1103 6 0.002809676732965727 1103 7 0.03133795563448416 1103 8 0.05406492539737684 1103 9 0.05239290253886253 1103 10-11 0.08904435382187614 1103 12-13 0.06854612943271832 1103 14-15 0.06625342943004142 1103 16-17 0.04004343652806597 1103 18-19 -0.002902760011572525 1103 20-21 0.04810772449104661 1103 22-23 0.015711233313083994 1103 24-25 0.02766302770010043 1103 26-27 0.009883824996627766 1103 28-29 -0.008302927775787339 1103 30-31 0.01101255071343843 1103 32-33 0.019974332923993643 1103 34-35 -0.0013894102417921772 1103 36-37 -3.6497574299687585E-4 1103 38-39 0.07361988792457197 1103 40-41 -0.0061077624223031535 1103 42-43 -0.07167612827313974 1103 44-45 -0.11705512747492719 1103 46-47 -0.14639474440777178 1103 48-49 -0.038566213387923653 1103 50-51 -0.15334778657778259 1103 52-53 -0.09765618578916957 1103 54-55 -0.07132499643950752 1103 56-57 -0.023390573334594933 1103 58-59 -0.07612195255781984 1103 60-61 -0.18504873296459579 1103 62-63 -0.16721229272467752 1103 64-65 -0.14814210895708158 1103 66-67 -0.19834920712442283 1103 68-69 -0.2183720023944602 1103 70-71 -0.14991195384063616 1103 72-73 -0.1530442416190283 1103 74-75 -0.15273692581903475 1103 76-77 -0.19608060626890023 1103 78-79 -0.25609983291958116 1103 80-81 -0.20943509433025298 1103 82-83 -0.29624359271087286 1103 84-85 -0.18965255324413022 1103 86-87 -0.37095222409926265 1103 88-89 -0.2308999791893882 1103 90-91 -0.20861419659038916 1103 92-93 -0.1564499928117229 1103 94-95 -0.15452198955607344 1103 96-97 -0.295104914428336 1103 98-99 -0.2753818936452106 1103 100-101 -0.11855218849067484 2204 1 0.035502745643569256 2204 2 -0.010474483955029257 2204 3 0.12476936561078134 2204 4 -0.022860422953392856 2204 5 0.07641802497219174 2204 6 0.07683737476800445 2204 7 0.06905735441984007 2204 8 0.08851614304342093 2204 9 0.14645829355302453 2204 10-11 0.16733264619770694 2204 12-13 0.1688889959843891 2204 14-15 0.10959361086194264 2204 16-17 0.17874266608063039 2204 18-19 0.13123669712506114 2204 20-21 0.1666249437187517 2204 22-23 0.0855113979367772 2204 24-25 0.08369792507579632 2204 26-27 0.1226689745565892 2204 28-29 0.13493892780464023 2204 30-31 0.07772741752708612 2204 32-33 0.08707182203783503 2204 34-35 0.09731519582433634 2204 36-37 0.12222400567126357 2204 38-39 0.11427866424324407 2204 40-41 0.08244258583071229 2204 42-43 0.016344919932009816 2204 44-45 0.04483622165582801 2204 46-47 0.060464380837224496 2204 48-49 0.053704419049829255 2204 50-51 0.09383494938895609 2204 52-53 0.12528264774227438 2204 54-55 0.014930210982058156 2204 56-57 0.054715864801302416 2204 58-59 0.11867194188960184 2204 60-61 0.08965957950640302 2204 62-63 0.04581396213691136 2204 64-65 0.1079518788046343 2204 66-67 0.042014970375355176 2204 68-69 0.07035306150854836 2204 70-71 0.07901625229673925 2204 72-73 0.04092259400701437 2204 74-75 0.011603434039351157 2204 76-77 -0.040952202699394036 2204 78-79 -0.025880819755805362 2204 80-81 0.0110057683052176 2204 82-83 0.04936696637307847 2204 84-85 0.027154839794846453 2204 86-87 0.07915259381390172 2204 88-89 0.04279840260780787 2204 90-91 0.07265615183401053 2204 92-93 0.06465829737891937 2204 94-95 0.05805513093414305 2204 96-97 0.008501443063678948 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-0.1428373563492613 1215 24-25 -0.13008364289618157 1215 26-27 -0.11513673625195509 1215 28-29 -0.1042507582087211 1215 30-31 -0.05566397644368237 1215 32-33 -0.10184754798623175 1215 34-35 -0.038354131391173496 1215 36-37 -0.12066127275020477 1215 38-39 -0.043798168362592094 1215 40-41 -0.005075952196605726 1215 42-43 0.05342448517490084 1215 44-45 0.12084868086920864 1215 46-47 0.06739320369988633 1215 48-49 0.12695386046492274 1215 50-51 0.07138056197188547 1215 52-53 0.07705361815431644 1215 54-55 0.07669551111293771 1215 56-57 0.06363795146476292 1215 58-59 0.1283396379364632 1215 60-61 0.06329804546131612 1215 62-63 0.026108387189957227 1215 64-65 -0.005929926347441494 1215 66-67 0.0520794083509486 1215 68-69 0.07740940644649896 1215 70-71 0.15843508699175146 1215 72-73 0.1938811683668007 1215 74-75 0.1397636973963543 1215 76-77 0.17582942985571748 1215 78-79 0.13375505872598126 1215 80-81 0.23977628308023924 1215 82-83 0.29314781741296514 1215 84-85 0.1780458649307235 1215 86-87 0.22516759191491786 1215 88-89 0.2154464357549557 1215 90-91 0.17425954677412392 1215 92-93 0.276160317527669 1215 94-95 0.27383856885463587 1215 96-97 0.2279345690002046 1215 98-99 0.23590603138039512 1215 100-101 0.1677668061912243 1212 1 -0.004811135780407483 1212 2 0.0021185474412632743 1212 3 -0.06238934208747082 1212 4 -0.042463206660187325 1212 5 0.016960361432659 1212 6 -0.12602366620373573 1212 7 -0.15809017116674084 1212 8 -0.2741499788270403 1212 9 -0.2620175437201553 1212 10-11 -0.21419638768667681 1212 12-13 -0.26916900100786734 1212 14-15 -0.24285186796603142 1212 16-17 -0.1566110151445983 1212 18-19 -0.1443312266345913 1212 20-21 -0.16472671555143137 1212 22-23 -0.1629495884012755 1212 24-25 -0.10391164924766372 1212 26-27 -0.08498378961877506 1212 28-29 -0.04251207303595095 1212 30-31 -0.0290595245351426 1212 32-33 -0.059937376193666125 1212 34-35 -0.04331924254898922 1212 36-37 -0.012506192229686519 1212 38-39 0.011913243765164339 1212 40-41 -0.022656024343845615 1212 42-43 0.1055054243715432 1212 44-45 0.04907436562173473 1212 46-47 0.04219389425706055 1212 48-49 0.11653480310169329 1212 50-51 -0.033778129671993895 1212 52-53 0.11767158682311418 1212 54-55 0.07541039006223826 1212 56-57 0.12900927684813013 1212 58-59 0.10095979535113031 1212 60-61 0.12639296367942876 1212 62-63 0.138945211089343 1212 64-65 0.1409804619437267 1212 66-67 0.18475542607558282 1212 68-69 0.1509416834990347 1212 70-71 0.12097286531056994 1212 72-73 0.12482745396167161 1212 74-75 0.1641598933323607 1212 76-77 0.2057744759043345 1212 78-79 0.21582412352447733 1212 80-81 0.12818099841988584 1212 82-83 0.1307557674252635 1212 84-85 0.10792163371865371 1212 86-87 0.043264850336193206 1212 88-89 0.06521253928367088 1212 90-91 0.08932818692344568 1212 92-93 0.08625556040195903 1212 94-95 0.09201409014612949 1212 96-97 0.07830548340317023 1212 98-99 0.17497527818449754 1212 100-101 -0.08248312126798396 1213 1 -0.3365321004675508 1213 2 -0.06908670668977379 1213 3 -0.22102902949558256 1213 4 -0.06208903233646623 1213 5 -0.08650020523986512 1213 6 -0.11987025228606996 1213 7 -0.14381985999677482 1213 8 -0.31377367697744063 1213 9 -0.2917771268623426 1213 10-11 -0.32671352531508546 1213 12-13 -0.22260067665111194 1213 14-15 -0.18927371045062102 1213 16-17 -0.22745412662545306 1213 18-19 -0.13251137917734468 1213 20-21 -0.21757049703136744 1213 22-23 -0.24043798405588745 1213 24-25 -0.31909710537784264 1213 26-27 -0.19220748631079232 1213 28-29 -0.20450713039872426 1213 30-31 -0.3998588008331936 1213 32-33 -0.3324037963504267 1213 34-35 -0.38664007881249063 1213 36-37 -0.3847248263966847 1213 38-39 -0.5404923274358566 1213 40-41 -0.39177580769651854 1213 42-43 -0.1399004794698584 1213 44-45 -0.20512898210027686 1213 46-47 -0.22603784864345045 1213 48-49 -0.20484490171696734 1213 50-51 -0.3490823111825492 1213 52-53 -0.3480081737787586 1213 54-55 -0.1499192938779217 1213 56-57 -0.21509004086375683 1213 58-59 -0.3123391715763546 1213 60-61 -0.15594026091846303 1213 62-63 -0.28854221731308627 1213 64-65 -0.3398256773410182 1213 66-67 -0.17734922246121698 1213 68-69 -0.15709562026511747 1213 70-71 -0.13820406527438678 1213 72-73 -0.12557117189682288 1213 74-75 -0.20973224582412797 1213 76-77 -0.17692876233159183 1213 78-79 -0.19117088772760837 1213 80-81 -0.12120638350724988 1213 82-83 -0.019904653494570823 1213 84-85 -0.007189316112274469 1213 86-87 -0.03433509244366206 1213 88-89 -0.08383007407302756 1213 90-91 -0.11379824666332183 1213 92-93 -0.10558969511883731 1213 94-95 -0.15078257478548807 1213 96-97 -0.16834445473343607 1213 98-99 -0.1394946429533519 1213 100-101 -0.849735629636001 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 60.0 3 57.0 4 71.0 5 114.0 6 111.0 7 73.0 8 46.0 9 47.0 10 57.0 11 51.0 12 70.0 13 108.0 14 695.0 15 5553.0 16 22119.0 17 38739.0 18 41965.0 19 38903.0 20 37911.0 21 39071.0 22 44028.0 23 49715.0 24 56643.0 25 64107.0 26 74262.0 27 82885.0 28 96098.0 29 110883.0 30 128604.0 31 152740.0 32 182746.0 33 222697.0 34 282698.0 35 379432.0 36 554955.0 37 955718.0 38 2369932.0 39 5295228.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.388623898171067 36.282886275877786 35.88536194962397 14.443127876327178 2 14.548816910150702 37.84070964559736 34.082501173080274 13.52797227117167 3 14.540465907989553 35.99422297678404 34.67089557100183 14.794415544224574 4 14.9691728537513 35.20691543525204 35.03302505010162 14.790886660895037 5 15.456745113963382 35.15836584806059 35.10377641637683 14.281112621599195 6 15.19754265657222 35.474925139128224 34.291284711432525 15.03624749286703 7 15.417056127156629 35.16163478416856 34.96936165674815 14.451947431926662 8 14.876152996665809 35.671812282564844 34.89040076482443 14.561633955944911 9 14.934453465818123 35.01392633246411 35.20094363988448 14.850676561833287 10-11 14.840783891815528 35.456673794999546 34.878181598943975 14.824360714240948 12-13 14.778624264135523 35.32284993215485 35.090249358161365 14.808276445548257 14-15 14.861801246554915 35.27684098012308 34.976122421039804 14.8852353522822 16-17 14.968095666696815 35.06899757065449 35.142614636465744 14.820292126182952 18-19 14.913283570460727 34.97670000156709 34.97585686232574 15.134159565646446 20-21 15.114798640605198 34.90410048020055 34.79032613971984 15.190774739474417 22-23 15.072632998624819 34.9746750222391 34.719756484655576 15.23293549448051 24-25 15.30254762800175 34.74461938675773 34.67663125494896 15.276201730291552 26-27 15.326982453178871 34.791873625595535 34.62462756221481 15.256516359010778 28-29 15.268932755343997 34.813650709182994 34.57569872789282 15.341717807580196 30-31 15.241153604239555 34.71970253402258 34.59701070956455 15.442133152173312 32-33 15.339990723227137 34.79958912355335 34.45360417106878 15.406815982150734 34-35 15.386365106092018 34.87057892911918 34.40501312010976 15.338042844679045 36-37 15.329248091974085 34.76538395420861 34.43035755073758 15.475010403079716 38-39 15.4178701322827 34.779062210206966 34.41006956489647 15.392998092613869 40-41 15.423789393270221 34.90527037768059 34.35439016839899 15.316550060650197 42-43 15.33884660508788 34.72588560251771 34.45820159918112 15.477066193213288 44-45 15.45733166961746 34.8348508891337 34.30720456222501 15.400612879023825 46-47 15.475237290309224 34.86048206115944 34.32982056193012 15.334460086601224 48-49 15.408777184920142 34.70362994147272 34.34149835909609 15.546094514511044 50-51 15.517153612877355 34.7384220116314 34.289989736533954 15.454434638957295 52-53 15.483949975364162 34.87249822410503 34.268607849766994 15.374943950763816 54-55 15.49280205005236 34.69820280043912 34.3045647402278 15.50443040928072 56-57 15.525057977006613 34.78657919371723 34.22555282655068 15.462810002725474 58-59 15.543783671822691 34.78357086323251 34.294952684799846 15.377692780144953 60-61 15.523371015831028 34.7415993600942 34.21059021491217 15.524439409162593 62-63 15.51458586093323 34.72244823035412 34.24827850967132 15.514687399041321 64-65 15.49579220854442 34.831232748602446 34.241372027673926 15.4316030151792 66-67 15.570783048761431 34.73407190476737 34.19199620001819 15.503148846453014 68-69 15.53331882708843 34.72919520403943 34.22806315628784 15.509422812584303 70-71 15.57713524520886 34.78801477102175 34.188416039821206 15.446433943948188 72-73 15.538669721834072 34.73219382653095 34.172197535115664 15.556938916519316 74-75 15.60455823289804 34.77498400307904 34.16631959212465 15.454138171898274 76-77 15.569248404236275 34.8159904441146 34.20630651881639 15.408454632832736 78-79 15.617119099851703 34.698931457994945 34.19309692637996 15.49085251577339 80-81 15.646907356460993 34.75796136441806 34.17064365115212 15.42448762796883 82-83 15.584781764708142 34.87250061228688 34.13370755942384 15.40901006358114 84-85 15.513253497859564 34.701461216921444 34.2289188476623 15.556366437556687 86-87 15.617779195463783 34.87764554382226 34.0894963155324 15.415078945181563 88-89 15.593541124636587 34.83819672147796 34.18756412901837 15.38069802486708 90-91 15.566011491962833 34.727928698397086 34.153387960074156 15.552671849565925 92-93 15.625226440286067 34.793728657761555 34.149449330341696 15.431595571610679 94-95 15.585524382470664 34.867611963793955 34.133281993527035 15.413581660208346 96-97 15.541416911720713 34.78234144861659 34.20530403002866 15.470937609634042 98-99 15.618885682405148 34.79951474267413 34.13248242116788 15.449117153752855 100-101 15.621702965218287 34.9141775167244 34.06213055325977 15.401988964797539 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1397.0 1 1503.5 2 1806.0 3 2137.5 4 2569.5 5 3147.0 6 3746.0 7 4574.0 8 5752.0 9 7416.0 10 9924.0 11 14057.5 12 20496.5 13 30273.5 14 45536.0 15 67713.5 16 97826.5 17 136543.5 18 182592.5 19 235413.0 20 290706.5 21 344800.0 22 398074.0 23 444979.0 24 482569.0 25 508326.5 26 520530.5 27 523579.0 28 525694.0 29 525343.0 30 518336.5 31 508401.5 32 493239.0 33 466949.5 34 429800.5 35 382771.5 36 335100.0 37 295308.5 38 260755.0 39 233822.5 40 213641.5 41 195673.0 42 180043.5 43 164180.5 44 147879.5 45 132952.5 46 120170.5 47 114189.0 48 104680.5 49 88510.0 50 74135.5 51 62420.0 52 52984.0 53 45030.5 54 39144.5 55 34602.5 56 30800.5 57 27352.5 58 24368.5 59 21461.5 60 17978.5 61 14717.5 62 11901.5 63 9643.5 64 7665.0 65 6076.0 66 4880.0 67 3907.5 68 3068.5 69 2387.5 70 1877.5 71 1465.0 72 1198.0 73 974.5 74 766.0 75 644.5 76 567.5 77 476.0 78 425.5 79 371.0 80 293.5 81 208.5 82 159.5 83 136.5 84 98.0 85 68.5 86 45.5 87 28.5 88 16.0 89 10.0 90 11.0 91 6.0 92 4.5 93 4.5 94 3.5 95 6.5 96 7.0 97 6.5 98 6.5 99 8.0 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.531798560744667E-4 2 3.8837721171995317E-4 3 0.0015358553372561783 4 0.0019065790393524974 5 0.008756140773322582 6 0.013346053275467484 7 0.013734430487187435 8 0.009727083802622464 9 0.0025950659146742328 10-11 0.006090460820153812 12-13 0.010495011471250553 14-15 0.03370496324892367 16-17 0.014612692590963238 18-19 0.02184180478184146 20-21 0.017172451486390204 22-23 0.030161021191979092 24-25 0.026043340072266406 26-27 0.02178443087556465 28-29 0.029812364376912315 30-31 0.032319162743468376 32-33 0.03648097763724015 34-35 0.0186641730495873 36-37 0.028413323739239302 38-39 0.028430977248862937 40-41 0.03168804977442345 42-43 0.03935408632848662 44-45 0.02771159673169984 46-47 0.02771159673169984 48-49 0.022137501068037333 50-51 0.03592930546150158 52-53 0.028355949832962492 54-55 0.030297835891562257 56-57 0.023999946333330746 58-59 0.025275412403638314 60-61 0.03332099941460962 62-63 0.029966832586119117 64-65 0.029110637369372853 66-67 0.02490910207894791 68-69 0.026793614231270864 70-71 0.029724096828794146 72-73 0.03633974956025107 74-75 0.0315556484522462 76-77 0.03367848298448821 78-79 0.034839201242242164 80-81 0.04110619715863232 82-83 0.04275238693103621 84-85 0.048066093327750116 86-87 0.04920915807588044 88-89 0.051437913665864254 90-91 0.05119517790853929 92-93 0.05157031498804151 94-95 0.052470643978846855 96-97 0.05304438304161497 98-99 0.05408594010940939 100-101 0.05753278786342397 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1329192E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.49010311880546 #Duplication Level Percentage of deduplicated Percentage of total 1 68.84891883520716 41.64678199959949 2 18.890599128558634 22.853885785250565 3 6.739685207381307 12.23052759548349 4 2.4688778284405526 5.973706977204061 5 1.0912370875025672 3.3004521975047614 6 0.5651077662071748 2.0510056230665885 7 0.3250025646351352 1.3761607054058895 8 0.1887897723338506 0.9135930237000341 9 0.11958035886115495 0.6510085414645522 >10 0.7351300562945519 7.808961820550501 >50 0.024385287259278972 0.9846041843453758 >100 0.0026861073186116246 0.2093115464246744 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.530701924726847E-5 0.0 0.0 2 3.530701924726847E-5 0.0 0.0 3 3.530701924726847E-5 8.826754811817118E-6 0.0 4 3.530701924726847E-5 8.826754811817118E-6 1.7653509623634236E-5 5 3.530701924726847E-5 8.826754811817118E-6 2.6480264435451354E-5 6 3.530701924726847E-5 8.826754811817118E-6 2.6480264435451354E-5 7 3.530701924726847E-5 8.826754811817118E-6 2.6480264435451354E-5 8 4.413377405908559E-5 8.826754811817118E-6 2.6480264435451354E-5 9 4.413377405908559E-5 8.826754811817118E-6 2.6480264435451354E-5 10-11 6.620066108862838E-5 1.7653509623634236E-5 4.8547151464994147E-5 12-13 7.061403849453694E-5 2.6480264435451354E-5 5.296052887090271E-5 14-15 9.709430292998829E-5 2.6480264435451354E-5 5.296052887090271E-5 16-17 1.1916118995953109E-4 2.6480264435451354E-5 7.502741590044551E-5 18-19 1.3240132217725675E-4 2.6480264435451354E-5 9.709430292998829E-5 20-21 2.0301536067179372E-4 2.6480264435451354E-5 9.709430292998829E-5 22-23 2.648026443545135E-4 2.6480264435451354E-5 1.0592105774180541E-4 24-25 3.1776317322541626E-4 2.6480264435451354E-5 1.2357456736543964E-4 26-27 3.5307019247268474E-4 3.530701924726847E-5 1.2357456736543964E-4 28-29 4.325109857790388E-4 3.530701924726847E-5 1.2357456736543964E-4 30-31 5.340186661149356E-4 3.972039665317703E-5 1.632949640186167E-4 32-33 6.487664786685582E-4 5.296052887090271E-5 1.721217188304338E-4 34-35 7.855811782517235E-4 5.296052887090271E-5 1.7653509623634237E-4 36-37 8.738487263698948E-4 5.296052887090271E-5 1.809484736422509E-4 38-39 0.00104155706779442 6.178728368271982E-5 1.8536185104815948E-4 40-41 0.0011960252770012196 6.620066108862838E-5 1.9418860585997659E-4 42-43 0.001301946334743025 7.944079330635406E-5 2.0301536067179372E-4 44-45 0.001460827921355733 7.944079330635406E-5 2.0742873807770226E-4 46-47 0.0016682566594334353 7.944079330635406E-5 2.339090025131536E-4 48-49 0.001853618510481595 7.944079330635406E-5 2.515625121367878E-4 50-51 0.00205222049374748 8.385417071226262E-5 2.7362939916633064E-4 52-53 0.002241995722201548 8.826754811817119E-5 2.7362939916633064E-4 54-55 0.00248031810212061 8.826754811817119E-5 3.221765506313248E-4 56-57 0.002670093330574678 8.826754811817119E-5 3.442434376608676E-4 58-59 0.0029084157104937404 8.826754811817119E-5 3.5307019247268474E-4 60-61 0.0032703126577782425 8.826754811817119E-5 3.795504569081361E-4 62-63 0.0035262885473209384 8.826754811817119E-5 3.972039665317703E-4 64-65 0.0038881854946054405 8.826754811817119E-5 4.325109857790388E-4 66-67 0.004205948667830857 8.826754811817119E-5 4.50164495402673E-4 68-69 0.004457511179967644 8.826754811817119E-5 4.678180050263073E-4 70-71 0.004788514485410787 9.709430292998829E-5 5.031250242735758E-4 72-73 0.005132757923071654 9.709430292998829E-5 5.119517790853928E-4 74-75 0.005596162550692053 1.0592105774180541E-4 5.119517790853928E-4 76-77 0.00615666148124244 1.2357456736543964E-4 5.119517790853928E-4 78-79 0.006924589149870529 1.5005483180089102E-4 5.119517790853928E-4 80-81 0.007652796421845442 1.5005483180089102E-4 5.119517790853928E-4 82-83 0.008460444487126708 1.632949640186167E-4 5.119517790853928E-4 84-85 0.009652056386722018 1.7653509623634237E-4 5.472587983326614E-4 86-87 0.010892215437782325 1.7653509623634237E-4 5.825658175799298E-4 88-89 0.0125825389842453 1.7653509623634237E-4 6.090460820153812E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGATCT 485 0.0 17.630245 32-33 TCCGATC 695 0.0 13.328382 32-33 GACCGGA 505 3.0464018E-4 9.406427 9 GACGGGA 600 1.7647799E-4 8.708784 9 GTGTGAC 2080 0.0 7.3056836 5 GTGACTG 1510 1.08975655E-8 6.9185357 7 GACAGGA 2400 9.640644E-11 5.937807 9 AGTCTCG 1090 0.0025035287 5.6654305 9 CAAGTGT 4310 0.0 5.398748 2 TGACAGG 1780 1.0173531E-5 5.336695 8 GTCTCGC 945 5.1481893E-6 5.2780747 10-11 CGATCTA 1185 4.769845E-7 5.010863 34-35 >>END_MODULE