##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l02n02 8-59796.34200000001b86.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10575190 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.714081070883836 38.0 32.0 38.0 32.0 38.0 2 34.82255373189513 38.0 32.0 38.0 32.0 38.0 3 34.68123021903153 38.0 32.0 38.0 32.0 38.0 4 35.2412611026374 38.0 32.0 38.0 32.0 38.0 5 35.07538455573848 38.0 32.0 38.0 32.0 38.0 6 37.00620461665464 38.0 38.0 40.0 32.0 40.0 7 37.334901689709596 40.0 38.0 40.0 32.0 40.0 8 37.49191135100173 40.0 38.0 40.0 32.0 40.0 9 37.536365587757764 40.0 38.0 40.0 32.0 40.0 10-11 37.55637444811866 40.0 38.0 40.0 32.0 40.0 12-13 37.558818612242426 40.0 38.0 40.0 32.0 40.0 14-15 37.5191788989134 40.0 38.0 40.0 32.0 40.0 16-17 37.513737105432625 40.0 38.0 40.0 32.0 40.0 18-19 37.49618909920295 40.0 38.0 40.0 32.0 40.0 20-21 37.46993358984567 40.0 38.0 40.0 32.0 40.0 22-23 37.45512666911895 40.0 38.0 40.0 32.0 40.0 24-25 37.40802340194361 40.0 38.0 40.0 32.0 40.0 26-27 37.38995247366714 40.0 38.0 40.0 32.0 40.0 28-29 37.312448570663975 40.0 38.0 40.0 32.0 40.0 30-31 37.27264640162493 40.0 38.0 40.0 32.0 40.0 32-33 37.27802630496473 40.0 38.0 40.0 32.0 40.0 34-35 37.19306329247985 40.0 38.0 40.0 32.0 40.0 36-37 37.19856054595709 40.0 38.0 40.0 32.0 40.0 38-39 37.15201783608616 40.0 38.0 40.0 32.0 40.0 40-41 37.03702491397318 39.0 38.0 40.0 32.0 40.0 42-43 36.774367552734276 38.0 38.0 40.0 32.0 40.0 44-45 36.6655953699177 38.0 38.0 40.0 32.0 40.0 46-47 36.58217100591101 38.0 38.0 40.0 32.0 40.0 48-49 36.49537615872623 38.0 38.0 40.0 32.0 40.0 50-51 36.42685601866255 38.0 38.0 40.0 29.5 40.0 52-53 36.35415283318787 38.0 38.0 40.0 27.0 40.0 54-55 36.29694341189142 38.0 38.0 40.0 27.0 40.0 56-57 36.27193747819189 38.0 38.0 40.0 27.0 40.0 58-59 36.201694532202254 38.0 38.0 40.0 27.0 40.0 60-61 36.162504550745666 38.0 38.0 40.0 27.0 40.0 62-63 36.081161520502235 38.0 38.0 40.0 27.0 40.0 64-65 36.059529143211606 38.0 38.0 40.0 27.0 40.0 66-67 35.99097902732717 38.0 38.0 40.0 27.0 40.0 68-69 35.92978603694118 38.0 38.0 40.0 27.0 40.0 70-71 35.816070822368204 38.0 38.0 40.0 27.0 40.0 72-73 35.73975413207706 38.0 38.0 40.0 27.0 40.0 74-75 35.64493919258188 38.0 38.0 40.0 27.0 40.0 76-77 35.55681642599329 38.0 38.0 40.0 27.0 40.0 78-79 35.46156518228041 38.0 38.0 40.0 27.0 40.0 80-81 35.38071211959313 38.0 38.0 40.0 27.0 40.0 82-83 35.27891810927274 38.0 38.0 40.0 27.0 40.0 84-85 35.16955969585416 38.0 38.0 40.0 27.0 40.0 86-87 35.09756496100779 38.0 38.0 40.0 27.0 40.0 88-89 35.02069688582427 38.0 38.0 40.0 27.0 40.0 90-91 34.89196704740057 38.0 35.0 40.0 27.0 40.0 92-93 34.78253648397806 38.0 32.0 40.0 20.0 40.0 94-95 34.672366312094624 38.0 32.0 39.0 13.0 40.0 96-97 34.58187224059331 38.0 32.0 38.0 13.0 40.0 98-99 34.433014725976555 38.0 32.0 38.0 13.0 40.0 100-101 32.44857874893974 38.0 29.5 38.0 13.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.019718195003250116 2201 2 0.35025142549838506 2201 3 0.3236178952605471 2201 4 0.2196432278706908 2201 5 0.34020863193199347 2201 6 0.335510014541164 2201 7 0.3213422126243799 2201 8 0.21278961541617747 2201 9 0.20241097843125289 2201 10-11 0.18665293053406629 2201 12-13 0.24665937220895273 2201 14-15 0.2471525337005005 2201 16-17 0.17448257649090237 2201 18-19 0.13508041232294943 2201 20-21 0.18895375760286726 2201 22-23 0.21733018860794573 2201 24-25 0.2099024255279005 2201 26-27 0.25228182304976343 2201 28-29 0.2272397576891123 2201 30-31 0.28579839071654334 2201 32-33 0.2618838143716502 2201 34-35 0.2410971940719051 2201 36-37 0.07601916506897766 2201 38-39 0.1966663937572548 2201 40-41 0.14522101749660266 2201 42-43 0.22389649207510587 2201 44-45 0.16717990524061577 2201 46-47 0.22667074204675686 2201 48-49 0.1666928142298758 2201 50-51 0.2037696343281965 2201 52-53 0.19413068644984577 2201 54-55 0.25903312631611186 2201 56-57 0.10569400404344975 2201 58-59 0.16759561166675496 2201 60-61 0.10060219198106779 2201 62-63 0.18703756887418166 2201 64-65 0.14718684642743796 2201 66-67 0.15163358506633529 2201 68-69 0.07139854841997106 2201 70-71 0.12451684057311496 2201 72-73 -0.014093259959409465 2201 74-75 0.08023899293895909 2201 76-77 0.10330132414728155 2201 78-79 0.14104542691107724 2201 80-81 0.21523146942364235 2201 82-83 0.16226822498540372 2201 84-85 0.03235922696094207 2201 86-87 0.14835246812376113 2201 88-89 0.08727102968370559 2201 90-91 0.033190893441748415 2201 92-93 -0.008206422386138001 2201 94-95 0.05934440542763042 2201 96-97 -0.11598463090262356 2201 98-99 -0.05450158541294314 2201 100-101 0.09002935001250734 1101 1 0.0368198648940421 1101 2 0.1690394730404563 1101 3 0.08619792794260661 1101 4 0.10662112950868874 1101 5 0.3365348028550841 1101 6 0.2828166473444895 1101 7 0.27054842502885634 1101 8 0.3094810989945813 1101 9 0.3088661579194252 1101 10-11 0.3381099070535214 1101 12-13 0.3051585165849957 1101 14-15 0.27713461963897146 1101 16-17 0.25738174947480275 1101 18-19 0.2238044929842431 1101 20-21 0.24361634061888537 1101 22-23 0.24709838864756506 1101 24-25 0.22290849224125964 1101 26-27 0.22623526577599762 1101 28-29 0.297189672849953 1101 30-31 0.3058883216358055 1101 32-33 0.2590799985580148 1101 34-35 0.3160160765414375 1101 36-37 0.30481841969777435 1101 38-39 0.29183558390184317 1101 40-41 0.2751928375548047 1101 42-43 0.179136691574719 1101 44-45 0.15410079206802152 1101 46-47 0.18046822375199412 1101 48-49 0.16478895550446282 1101 50-51 0.20948701645238543 1101 52-53 0.2547367922579227 1101 54-55 0.21315033193144473 1101 56-57 0.25419935917660297 1101 58-59 0.25304206897375536 1101 60-61 0.16861201648875834 1101 62-63 0.22177570555463433 1101 64-65 0.1917727497465549 1101 66-67 0.22982656011040348 1101 68-69 0.19451786828430784 1101 70-71 0.21306483426652534 1101 72-73 0.23632353779311188 1101 74-75 0.21393260643640133 1101 76-77 0.2334573826037385 1101 78-79 0.18585380953469866 1101 80-81 0.25101982474123474 1101 82-83 0.2691907179911439 1101 84-85 0.16038161963829367 1101 86-87 0.17652604648014858 1101 88-89 0.24876915711481473 1101 90-91 0.11051588931329803 1101 92-93 0.13399797249547163 1101 94-95 0.10838126596474495 1101 96-97 0.13019923904896302 1101 98-99 0.11505283721265869 1101 100-101 0.1317898962457349 2202 1 -0.12705596344991932 2202 2 -0.04742526864794172 2202 3 -0.033462485287607535 2202 4 0.12458388147764055 2202 5 0.05733224243931545 2202 6 0.0019049964568367272 2202 7 0.11595548137871248 2202 8 0.08402276112015272 2202 9 0.23447566511454454 2202 10-11 0.17937168168380424 2202 12-13 0.15829109138803688 2202 14-15 0.14463352235200944 2202 16-17 0.15473554404817946 2202 18-19 0.15093411926511635 2202 20-21 0.1750607689298178 2202 22-23 0.17901132697640776 2202 24-25 0.16087427090660356 2202 26-27 0.15223458304129167 2202 28-29 0.16686831051140416 2202 30-31 0.19059089516985495 2202 32-33 0.19218465113751648 2202 34-35 0.1501884719701252 2202 36-37 0.1578099011877825 2202 38-39 0.12165448202372176 2202 40-41 0.11927550248456953 2202 42-43 0.10920003266884493 2202 44-45 0.030877604750322973 2202 46-47 0.0016475561955573426 2202 48-49 0.09822555899229002 2202 50-51 0.22198452239710775 2202 52-53 0.11238638044856941 2202 54-55 0.08347190406512084 2202 56-57 0.06760818160235971 2202 58-59 0.06871158001138156 2202 60-61 -0.024983149772914715 2202 62-63 0.06635924055009212 2202 64-65 0.0495857493638141 2202 66-67 0.06626522002864732 2202 68-69 -0.016735574412486187 2202 70-71 -0.008968116128215797 2202 72-73 -0.10431041637643546 2202 74-75 0.02187915696385545 2202 76-77 -0.09390475850603508 2202 78-79 -0.06578516019887104 2202 80-81 0.003860153947272238 2202 82-83 -0.06125360571428473 2202 84-85 0.009457374714592959 2202 86-87 0.005348485856288221 2202 88-89 -0.02290786196013528 2202 90-91 0.028851098228123817 2202 92-93 0.035035935144243524 2202 94-95 0.011142238092126888 2202 96-97 -0.004831615195456607 2202 98-99 -0.05337747558507999 2202 100-101 -0.022187452012879305 1102 1 -0.28390323198500766 1102 2 -0.16750816453166806 1102 3 -0.25745966523327724 1102 4 -0.0829458249123789 1102 5 -0.06755223504335817 1102 6 -0.24300021851992426 1102 7 0.03862277834312522 1102 8 0.07546149207529851 1102 9 0.19051990943318486 1102 10-11 0.07601684959738719 1102 12-13 0.1866785261898798 1102 14-15 0.16052711361846406 1102 16-17 0.171639004614498 1102 18-19 0.14921733543971527 1102 20-21 0.17758363869103988 1102 22-23 0.18653198199609022 1102 24-25 0.2189622124287922 1102 26-27 0.1607148888105172 1102 28-29 0.02379010703266715 1102 30-31 0.14004382121275682 1102 32-33 0.08512795151631991 1102 34-35 0.121540615364502 1102 36-37 0.1475568204361224 1102 38-39 0.15228507623969278 1102 40-41 0.16143282528211245 1102 42-43 0.10030270599406066 1102 44-45 -0.025273717452620303 1102 46-47 0.04451266217683525 1102 48-49 -0.06019176953858363 1102 50-51 -0.03565210330496882 1102 52-53 0.007755183869875282 1102 54-55 -0.07373959694443499 1102 56-57 -0.07250019498611238 1102 58-59 -0.054186995635078006 1102 60-61 -0.02586226556029203 1102 62-63 -0.14979274789683927 1102 64-65 -0.16006775414092544 1102 66-67 -0.1658014192320607 1102 68-69 -0.2927510537765059 1102 70-71 -0.11209350454295475 1102 72-73 -0.15852348781896808 1102 74-75 -0.06167376923256995 1102 76-77 -0.13290319011357354 1102 78-79 -0.17121846036069144 1102 80-81 -0.09630591810079636 1102 82-83 -0.16529462002967676 1102 84-85 -0.06898330305998712 1102 86-87 -0.1080372165138499 1102 88-89 -0.25433513750329695 1102 90-91 -0.4469001781423856 1102 92-93 -0.19742539256559866 1102 94-95 -0.19589956392059094 1102 96-97 -0.22046212718967695 1102 98-99 -0.18028780355619745 1102 100-101 -0.2497780254996229 2203 1 -0.38319978356170736 2203 2 -0.28673445973751654 2203 3 -0.2732574410961064 2203 4 -0.15688074836024413 2203 5 -0.22720329029110076 2203 6 -0.18298111765442115 2203 7 0.05753344197054844 2203 8 0.10364661222342164 2203 9 0.10167850952941393 2203 10-11 0.06400233254660748 2203 12-13 0.13148336242182523 2203 14-15 0.11118807943410047 2203 16-17 0.18871707335733134 2203 18-19 0.15905998860132087 2203 20-21 0.16295195994966605 2203 22-23 0.10489995692169174 2203 24-25 0.09683146175231627 2203 26-27 0.0493855000205059 2203 28-29 0.051803147077322365 2203 30-31 0.07061879652635383 2203 32-33 0.07768741246511013 2203 34-35 0.03339754500814962 2203 36-37 0.016810489606633894 2203 38-39 -0.016318544344954944 2203 40-41 0.05578077385398217 2203 42-43 -0.05799180935455439 2203 44-45 -0.11583438560965931 2203 46-47 -0.1961880792930657 2203 48-49 -0.19150836118610925 2203 50-51 -0.13169412299847494 2203 52-53 -0.19514603174010148 2203 54-55 -0.09628712340673218 2203 56-57 -0.2294367274010085 2203 58-59 -0.10692432904235005 2203 60-61 -0.2524109353594355 2203 62-63 -0.26291573184938954 2203 64-65 -0.33797588226446607 2203 66-67 -0.3045084593897016 2203 68-69 -0.1902473141323 2203 70-71 -0.15621072794393598 2203 72-73 -0.1485280312287216 2203 74-75 -0.17207597169162625 2203 76-77 -0.2380638634655341 2203 78-79 -0.2811752351216086 2203 80-81 -0.2543772968455187 2203 82-83 -0.27344009824630433 2203 84-85 -0.30364855354053333 2203 86-87 -0.4120872945470637 2203 88-89 -0.3646594869472892 2203 90-91 -0.2716409183205002 2203 92-93 -0.34416615818458496 2203 94-95 -0.2436907297686446 2203 96-97 -0.35654109905487985 2203 98-99 -0.41923768588794275 2203 100-101 -0.16892286789774147 1103 1 -0.3965907615590325 1103 2 -0.18978594821633266 1103 3 -0.2872098593229566 1103 4 -0.14026658526753266 1103 5 -0.06132450732633998 1103 6 -0.2112184313835428 1103 7 0.07164010098230733 1103 8 0.12612311078083138 1103 9 0.21101317045446422 1103 10-11 0.1861177314383582 1103 12-13 0.1415056316761678 1103 14-15 0.14760125282587921 1103 16-17 0.10475318919819188 1103 18-19 0.07696911006019747 1103 20-21 0.12379757696898963 1103 22-23 0.13161465488016688 1103 24-25 0.15663241811525097 1103 26-27 0.10191496940121425 1103 28-29 0.035323271013183444 1103 30-31 0.09069756360894843 1103 32-33 0.0868083876570509 1103 34-35 0.11044254163136458 1103 36-37 0.0954996501522416 1103 38-39 0.14282536756188335 1103 40-41 0.06898287156099059 1103 42-43 -0.03529708595498704 1103 44-45 -0.12485124692447869 1103 46-47 -0.18900178312871674 1103 48-49 -0.09123740103157019 1103 50-51 -0.10622334167054248 1103 52-53 -0.0793027680783851 1103 54-55 -0.04656082669752948 1103 56-57 -0.05837972236201239 1103 58-59 -0.09769787918368422 1103 60-61 -0.15836387650674055 1103 62-63 -0.20255466910020203 1103 64-65 -0.11777295054164227 1103 66-67 -0.2104319953559255 1103 68-69 -0.21631272683000446 1103 70-71 -0.1180000458102981 1103 72-73 -0.06899906140599654 1103 74-75 -0.14232731923036823 1103 76-77 -0.2298354045694495 1103 78-79 -0.22305713087234835 1103 80-81 -0.17980410041786854 1103 82-83 -0.2900763371187125 1103 84-85 -0.17527690427944975 1103 86-87 -0.2500567100329647 1103 88-89 -0.25190806523877995 1103 90-91 -0.21516580582834166 1103 92-93 -0.18602884659230057 1103 94-95 -0.13997909396400843 1103 96-97 -0.1479887493309775 1103 98-99 -0.193249943287789 1103 100-101 -0.069866985065552 2204 1 -0.10014941816408651 2204 2 -0.07569097467710151 2204 3 -0.08298095687712959 2204 4 -0.016618436400307246 2204 5 -0.034518758851206144 2204 6 -0.10866425261895074 2204 7 0.08083237274379229 2204 8 0.12030634135338403 2204 9 0.15788457970008096 2204 10-11 0.18968536349616727 2204 12-13 0.25141518462557855 2204 14-15 0.18537273181981107 2204 16-17 0.2040314933241092 2204 18-19 0.2520082740507874 2204 20-21 0.26186116669281745 2204 22-23 0.22119335929020423 2204 24-25 0.18060744655082317 2204 26-27 0.20076696924725468 2204 28-29 0.19253395254254713 2204 30-31 0.20068623354367077 2204 32-33 0.16332449178330677 2204 34-35 0.10592566711773799 2204 36-37 0.1872800115490847 2204 38-39 0.21117020014200705 2204 40-41 0.11198583009714724 2204 42-43 0.043319680082042566 2204 44-45 0.01910359200810774 2204 46-47 0.05457596533784681 2204 48-49 0.0012607545332770087 2204 50-51 0.034349327885593084 2204 52-53 0.11402879411479461 2204 54-55 -0.016721378590709435 2204 56-57 -0.004289875735928206 2204 58-59 0.12012235398036353 2204 60-61 -0.031213084382272882 2204 62-63 0.0666098929043244 2204 64-65 0.0469135535742069 2204 66-67 0.03393951758980762 2204 68-69 0.029829327511158965 2204 70-71 0.027395341398104733 2204 72-73 0.07906167771132999 2204 74-75 0.09103102103244964 2204 76-77 0.11435404376327796 2204 78-79 -0.03255161329600753 2204 80-81 -0.03634953171707167 2204 82-83 -0.03510784055018945 2204 84-85 0.09440600540877853 2204 86-87 0.014672867423300318 2204 88-89 0.06811851392367885 2204 90-91 0.06402783093577114 2204 92-93 0.06269550614489106 2204 94-95 0.07861623596048872 2204 96-97 0.0486237663433684 2204 98-99 0.03925584362008294 2204 100-101 0.09594914534498855 2205 1 0.016792196233275547 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1215 60-61 -0.03146296921354974 1215 62-63 -0.06790202246054378 1215 64-65 -0.12019086970705217 1215 66-67 0.025518357010632542 1215 68-69 0.01767333115569869 1215 70-71 0.0776898791842271 1215 72-73 0.09156787022949686 1215 74-75 0.07740583099823795 1215 76-77 0.10263872300843246 1215 78-79 0.13804979746842605 1215 80-81 0.19161006404804937 1215 82-83 0.28453356660354956 1215 84-85 0.1828464071952709 1215 86-87 0.17877635853697882 1215 88-89 0.12046189918592631 1215 90-91 0.1332889950850884 1215 92-93 0.1786139826034372 1215 94-95 0.22598017864399367 1215 96-97 0.18697612475581593 1215 98-99 0.1989571489148645 1215 100-101 0.22925825588699666 1212 1 0.31654658686741755 1212 2 0.18361335260956224 1212 3 0.2112891482368866 1212 4 -0.1337609831539126 1212 5 0.016508725765284282 1212 6 -0.053482385226665485 1212 7 -0.29071271364757223 1212 8 -0.417220068583795 1212 9 -0.42745122661986557 1212 10-11 -0.434863168694946 1212 12-13 -0.5310517569103297 1212 14-15 -0.45889511646223013 1212 16-17 -0.3513040049632963 1212 18-19 -0.3477331787356377 1212 20-21 -0.3770730939690239 1212 22-23 -0.40380754668787233 1212 24-25 -0.2638184043086369 1212 26-27 -0.2659188982951832 1212 28-29 -0.18939595493517913 1212 30-31 -0.23304869733642164 1212 32-33 -0.2110423579973002 1212 34-35 -0.19785496995090313 1212 36-37 -0.1548497784922489 1212 38-39 -0.21887044284605395 1212 40-41 -0.19967232019525483 1212 42-43 -0.056607491450058944 1212 44-45 -0.0196198178056477 1212 46-47 -0.012332605139071973 1212 48-49 0.06384206268557335 1212 50-51 -0.13444768764411208 1212 52-53 -0.06445902272466952 1212 54-55 -0.006328707947986345 1212 56-57 0.006779858019022811 1212 58-59 0.01378995121723392 1212 60-61 0.003965686187626716 1212 62-63 -0.006374923822527023 1212 64-65 0.0307395731132587 1212 66-67 0.003100469923673188 1212 68-69 0.028494456701473325 1212 70-71 -0.03215536865612734 1212 72-73 0.02945089941719914 1212 74-75 0.05443225151835662 1212 76-77 0.09444974587898258 1212 78-79 0.05484808546492559 1212 80-81 -0.018797477461802714 1212 82-83 -0.040776281725932506 1212 84-85 -0.10562421234075003 1212 86-87 -0.11168067059148257 1212 88-89 -0.09463491789425404 1212 90-91 -0.10219631501897908 1212 92-93 -0.10874375389331448 1212 94-95 -0.011023649110647682 1212 96-97 -0.02144879060155347 1212 98-99 -0.07371132735815422 1212 100-101 -0.15872470092302393 1213 1 0.2173257599817191 1213 2 0.26842833459505755 1213 3 0.18254510895985732 1213 4 -0.06046272541045994 1213 5 0.06543939461441539 1213 6 0.18248484731783066 1213 7 -0.07572273873478963 1213 8 -0.4241378781396463 1213 9 -0.36469547580850303 1213 10-11 -0.4409997947916793 1213 12-13 -0.35191961415327455 1213 14-15 -0.28335279566001503 1213 16-17 -0.3469580461321371 1213 18-19 -0.2851014889803025 1213 20-21 -0.30314153932111054 1213 22-23 -0.42874852278902154 1213 24-25 -0.48383883434554065 1213 26-27 -0.37709533550931695 1213 28-29 -0.44533981245581344 1213 30-31 -0.6917728901809213 1213 32-33 -0.5320894848882958 1213 34-35 -0.5784639755671392 1213 36-37 -0.6188108074588712 1213 38-39 -0.7706064438286546 1213 40-41 -0.5921139033652949 1213 42-43 -0.20288092010319048 1213 44-45 -0.3248573193023958 1213 46-47 -0.37156501348151494 1213 48-49 -0.30050207193063727 1213 50-51 -0.4913915026004645 1213 52-53 -0.5236238067977226 1213 54-55 -0.2194433396308213 1213 56-57 -0.26059081065464795 1213 58-59 -0.3720843733687076 1213 60-61 -0.3221246358510683 1213 62-63 -0.44017429771074035 1213 64-65 -0.4831708097130516 1213 66-67 -0.3156844091897071 1213 68-69 -0.26854476510128933 1213 70-71 -0.29875583679108075 1213 72-73 -0.27384408828203277 1213 74-75 -0.356632628924352 1213 76-77 -0.37139896706167264 1213 78-79 -0.3575448409064279 1213 80-81 -0.22991341043474733 1213 82-83 -0.19723024038284365 1213 84-85 -0.10038374168148323 1213 86-87 -0.15482116212923813 1213 88-89 -0.10244224554996606 1213 90-91 -0.18564845633074611 1213 92-93 -0.3174669799892271 1213 94-95 -0.40819227875920205 1213 96-97 -0.3375851217784316 1213 98-99 -0.3022360656643741 1213 100-101 -0.8965016895738387 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56.0 3 40.0 4 85.0 5 111.0 6 100.0 7 82.0 8 38.0 9 56.0 10 64.0 11 60.0 12 80.0 13 124.0 14 399.0 15 2974.0 16 15788.0 17 35481.0 18 47820.0 19 52362.0 20 56469.0 21 63045.0 22 71503.0 23 80178.0 24 89359.0 25 99367.0 26 111183.0 27 124471.0 28 141022.0 29 159317.0 30 181397.0 31 211255.0 32 246137.0 33 294944.0 34 365690.0 35 479862.0 36 692111.0 37 1201875.0 38 2835650.0 39 2914635.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.771648970101715 31.05074343350365 29.531924076147774 19.64568352024686 2 20.932758665198698 31.884644251280857 28.460786407980905 18.721810675539547 3 20.736672181853066 30.09530079452159 29.353128871438162 19.814898152187183 4 20.881395885644103 29.223781210458117 29.708508380267045 20.186314523630735 5 21.071518547295582 29.986001862635547 29.35635047507798 19.58612911499089 6 21.283615531961296 30.202563743183834 28.263092459653414 20.250728265201452 7 21.38192868598519 30.211432418174706 29.084587910024943 19.322050985815164 8 20.492676580017058 30.15155438381004 29.560424574703724 19.795344461469178 9 20.67164405633838 29.618198284293722 29.635399413724233 20.074758245643665 10-11 20.854344741249797 29.848749070110888 29.08817034466881 20.208735843970505 12-13 20.608881977841513 29.94215320947196 29.563395869275283 19.88556894341124 14-15 20.81300243909245 29.6738748462265 29.367978504581398 20.145144210099648 16-17 20.918416154506396 29.669035444852742 29.330999973566225 20.081548427074633 18-19 20.655423536458706 29.513666062064747 29.50284584107687 20.328064560399675 20-21 20.944693940628607 29.490056470501003 29.134023145830994 20.431226443039396 22-23 20.892835530672855 29.45057202747639 29.21015670436577 20.44643573748499 24-25 21.094773931816356 29.235154316563417 29.11597106212989 20.554100689490333 26-27 21.229247289941885 29.445581109931148 28.858572099292946 20.466599500834022 28-29 21.081867963867737 29.438334353718094 28.960023382536455 20.519774299877717 30-31 21.32917050298707 29.15694314044629 28.877005853436266 20.636880503130374 32-33 21.283442628704154 29.353145399467856 28.93201065801322 20.431401313814767 34-35 21.41259703451311 29.21491575233201 28.70267112865194 20.669816084502937 36-37 21.387349503489386 29.389270213794656 28.67767759771789 20.545702684998073 38-39 21.317663579508743 29.164791256104078 28.917986674302465 20.599558490084707 40-41 21.47378508549846 29.314767496008926 28.64997444595298 20.561472972539637 42-43 21.358453283314415 29.287274899266237 28.776246261683212 20.578025555736133 44-45 21.409634956462668 29.04149196699152 28.727819994928204 20.821053081617613 46-47 21.554268516550998 29.365863057362045 28.47216648750896 20.607701938578 48-49 21.353860579820342 29.09447105804753 28.731412402729852 20.820255959402274 50-51 21.460929605768616 29.208101552433046 28.50075004214198 20.830218799656354 52-53 21.427801543981083 29.264618603396652 28.700264484240122 20.607315368382142 54-55 21.53410680675657 28.998069217219495 28.5900338615334 20.877790114490537 56-57 21.617564617960642 29.31673363353584 28.386152523291013 20.679549225212508 58-59 21.40275882858109 29.19869572880837 28.634576258233096 20.763969184377444 60-61 21.51080462344767 29.14239347812145 28.34427514723774 21.00252675119314 62-63 21.414246274083226 29.199898316908364 28.6260320538211 20.759823355187315 64-65 21.526795509195736 29.051788930610538 28.505586642379026 20.915828917814693 66-67 21.54234050315121 29.164538131418293 28.34353438087692 20.949586984553573 68-69 21.30675811145001 29.15454641631625 28.568905819417996 20.969789652815745 70-71 21.643975634547726 29.08128325169329 28.25277066308438 21.0219704506746 72-73 21.498139422138927 29.178179152304885 28.4377507523309 20.885930673225296 74-75 21.597702344317472 29.085330537724868 28.402557781210596 20.914409336747063 76-77 21.52784422311362 29.276718076162016 28.303327736396028 20.892109964328334 78-79 21.523774064374983 29.096676175308634 28.49143200602075 20.88811775429563 80-81 21.745166963900893 29.080419548665624 28.205702356342783 20.9687111310907 82-83 21.515535570169867 29.364612061760276 28.418692463719935 20.701159904349918 84-85 21.58619809484309 29.083708985995145 28.311673702900876 21.01841921626089 86-87 21.475496712675692 29.30038202281314 28.259210324090954 20.96491094042021 88-89 21.490495877972428 29.091281597346825 28.416691446673347 21.0015310780074 90-91 21.60735456387683 29.014043811423758 28.240734915218574 21.13786670948084 92-93 21.43584796689059 29.207480128664198 28.368292355367846 20.98837954907736 94-95 21.568842273969796 28.951370099703606 28.352192885666433 21.127594740660165 96-97 21.609491373916658 29.204671019374306 28.177376762770436 21.0084608439386 98-99 21.464712263725186 29.188321229485286 28.274747037807185 21.07221946898234 100-101 21.872711618909857 28.905035771303794 27.804441406634194 21.417811203152155 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 181.0 1 228.5 2 305.0 3 360.0 4 451.5 5 578.0 6 678.5 7 780.0 8 969.0 9 1280.0 10 1635.5 11 2206.0 12 3139.5 13 4304.5 14 5923.0 15 8571.5 16 12005.5 17 16513.0 18 22399.0 19 28839.0 20 35570.5 21 42610.5 22 50457.5 23 58740.5 24 65786.0 25 71830.0 26 79276.0 27 86935.0 28 95468.5 29 108978.0 30 123983.5 31 143906.0 32 175350.0 33 218941.5 34 268669.0 35 328102.5 36 408584.5 37 491353.5 38 555158.0 39 593234.5 40 604034.5 41 585376.5 42 536305.5 43 468628.5 44 402515.0 45 355040.5 46 320201.5 47 297745.5 48 286162.5 49 273423.5 50 260382.0 51 244395.5 52 223929.0 53 204840.0 54 189652.0 55 174372.0 56 159692.5 57 146254.5 58 132066.5 59 116418.0 60 98874.0 61 81655.5 62 66055.0 63 52483.0 64 41442.5 65 32937.5 66 26234.5 67 20181.0 68 15119.0 69 11230.5 70 8196.0 71 6102.5 72 4752.0 73 3687.5 74 2777.5 75 2089.0 76 1566.0 77 1143.0 78 797.5 79 621.0 80 443.0 81 297.0 82 200.5 83 136.0 84 102.0 85 88.0 86 64.5 87 45.5 88 38.5 89 28.0 90 18.0 91 19.0 92 17.0 93 12.5 94 9.5 95 10.0 96 8.5 97 7.5 98 10.0 99 14.5 100 28.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.430144517498031E-4 2 4.0661207978296375E-4 3 0.0013900459471650156 4 0.001598080034495834 5 0.008756343857651729 6 0.01354112786626056 7 0.014221966697525058 8 0.00975868991479113 9 0.0028651967482380934 10-11 0.0063970481854226735 12-13 0.010756307924491192 14-15 0.03437290488397845 16-17 0.01508719937892369 18-19 0.022628435044665864 20-21 0.01779637056166367 22-23 0.030982894869973972 24-25 0.02662363513090545 26-27 0.022259647344397594 28-29 0.030235863374558754 30-31 0.033441479538429096 32-33 0.03772036247102889 34-35 0.019091855560041948 36-37 0.0289309222813018 38-39 0.02894037837618047 40-41 0.032897754082905364 42-43 0.040245139803634734 44-45 0.02835882854114205 46-47 0.028685063814456287 48-49 0.022510233858682446 50-51 0.035781863020900806 52-53 0.02935644655084211 54-55 0.03131858623816688 56-57 0.02410358584573894 58-59 0.02548890374546462 60-61 0.03320980521390159 62-63 0.029573936733051604 64-65 0.028443933395050115 66-67 0.024425093071613845 68-69 0.026812757028478924 70-71 0.029994732955152578 72-73 0.03666600789205679 74-75 0.03187649583600862 76-77 0.03436817683653911 78-79 0.0354981801745406 80-81 0.041625729655921075 82-83 0.043280546259688954 84-85 0.04862323986613952 86-87 0.04984780415292775 88-89 0.05213145106612742 90-91 0.05182885603000986 92-93 0.052060530354537365 94-95 0.05307233250655544 96-97 0.05368225062622989 98-99 0.0550722965733949 100-101 0.05837720173349131 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.057519E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.18147606557284 #Duplication Level Percentage of deduplicated Percentage of total 1 39.342619074894344 7.5464950614203525 2 5.187161478320208 1.9899482748932102 3 6.162097553082407 3.545943801845255 4 6.683552303302596 5.12801594155212 5 6.30959571011262 6.051367954848316 6 6.255715305533228 7.199631204367399 7 5.466165063659529 7.339438003733899 8 4.735972081082409 7.267434809640274 9 3.871844469091717 6.684092281215302 >10 15.658883358968332 42.37205182405528 >50 0.2817245320334974 3.5846453953050257 >100 0.04410364540339518 1.1551510381160526 >500 9.663205112489062E-5 0.01738625562518504 >1k 4.687924646184762E-4 0.11839815338228368 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.456094878673574E-6 0.0 0.0 2 1.891218975734715E-5 0.0 0.0 3 1.891218975734715E-5 0.0 0.0 4 1.891218975734715E-5 0.0 0.0 5 1.891218975734715E-5 0.0 0.0 6 1.891218975734715E-5 0.0 0.0 7 2.8368284636020723E-5 0.0 0.0 8 2.8368284636020723E-5 0.0 0.0 9 2.8368284636020723E-5 0.0 0.0 10-11 3.309633207535751E-5 0.0 0.0 12-13 7.56487590293886E-5 4.728047439336787E-6 0.0 14-15 1.3238532830143004E-4 9.456094878673574E-6 0.0 16-17 1.512975180587772E-4 9.456094878673574E-6 0.0 18-19 2.22218229648829E-4 9.456094878673574E-6 0.0 20-21 2.931389412388808E-4 9.456094878673574E-6 0.0 22-23 4.207962221009741E-4 1.465694706194404E-4 0.0 24-25 6.193742145531192E-4 1.512975180587772E-4 1.891218975734715E-5 26-27 8.746887762773057E-4 1.512975180587772E-4 1.891218975734715E-5 28-29 0.0010969070059261346 1.512975180587772E-4 1.891218975734715E-5 30-31 0.0012765728086209325 1.512975180587772E-4 1.891218975734715E-5 32-33 0.0015507995601024662 1.512975180587772E-4 1.891218975734715E-5 34-35 0.0018486665487806838 1.512975180587772E-4 1.891218975734715E-5 36-37 0.002070884778429513 1.9857799245214506E-4 1.891218975734715E-5 38-39 0.0023687517671077303 2.3640237196683936E-4 1.891218975734715E-5 40-41 0.0027848199417693674 3.2623527331423834E-4 1.891218975734715E-5 42-43 0.003338001492171772 3.4987551051092225E-4 9.456094878673574E-5 44-45 0.0037824379514694298 3.4987551051092225E-4 9.456094878673574E-5 46-47 0.00431197926467515 3.4987551051092225E-4 9.456094878673574E-5 48-49 0.004728047439336787 3.4987551051092225E-4 9.456094878673574E-5 50-51 0.005040098570333015 3.4987551051092225E-4 9.456094878673574E-5 52-53 0.005508175266827358 3.4987551051092225E-4 9.456094878673574E-5 54-55 0.006155917766016497 3.6405965282893264E-4 9.456094878673574E-5 56-57 0.006581442035556808 3.687877002682694E-4 9.456094878673574E-5 58-59 0.007191360155231254 3.687877002682694E-4 9.456094878673574E-5 60-61 0.007824918512102383 3.687877002682694E-4 9.456094878673574E-5 62-63 0.008340275682990093 3.687877002682694E-4 9.456094878673574E-5 64-65 0.008945465755225201 3.687877002682694E-4 9.456094878673574E-5 66-67 0.00949391925818827 3.687877002682694E-4 9.456094878673574E-5 68-69 0.010188942231770777 3.687877002682694E-4 1.1347313854408289E-4 70-71 0.01096434201182201 3.687877002682694E-4 1.3238532830143004E-4 72-73 0.011564804036617781 3.78243795146943E-4 1.3238532830143004E-4 74-75 0.012411124528259068 3.78243795146943E-4 1.3238532830143004E-4 76-77 0.01303995483769086 3.78243795146943E-4 1.3238532830143004E-4 78-79 0.01371606562151602 3.78243795146943E-4 1.3711337574076683E-4 80-81 0.0145103775913246 3.8769989002561655E-4 1.4184142318010362E-4 82-83 0.015649837024204766 3.8769989002561655E-4 1.4184142318010362E-4 84-85 0.016491429468406715 3.924279374649533E-4 1.512975180587772E-4 86-87 0.017304653627972642 3.971559849042901E-4 1.7020970781612434E-4 88-89 0.018765620286727708 4.066120797829637E-4 1.7020970781612434E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCTA 485 0.0 14.691156 34-35 TCCGATC 855 0.0 13.611481 32-33 GCCTAAG 2755 0.0 13.099901 1 CCGATCT 890 0.0 9.873864 32-33 CTTCCGA 1175 0.0 9.297796 30-31 GACGTGT 1215 0.0 8.014394 20-21 TTCCGAT 1225 0.0 7.3672867 30-31 GCCTAAA 2535 0.0 6.743736 1 CTAAGAG 2980 0.0 6.374143 3 AAGTGAC 3545 0.0 6.2959275 5 AGTTCCG 545 3.792976E-4 6.100709 14-15 CCTAAGA 3055 0.0 6.0622168 2 TCAGACG 1625 0.0 5.846216 18-19 AGACGTG 1880 0.0 5.684834 20-21 CAGACGT 1515 4.7293724E-11 5.33009 18-19 >>END_MODULE