FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l02n02 8-59796.34200000001b86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l02n02 8-59796.34200000001b86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10575190
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA4850.014.69115634-35
TCCGATC8550.013.61148132-33
GCCTAAG27550.013.0999011
CCGATCT8900.09.87386432-33
CTTCCGA11750.09.29779630-31
GACGTGT12150.08.01439420-21
TTCCGAT12250.07.367286730-31
GCCTAAA25350.06.7437361
CTAAGAG29800.06.3741433
AAGTGAC35450.06.29592755
AGTTCCG5453.792976E-46.10070914-15
CCTAAGA30550.06.06221682
TCAGACG16250.05.84621618-19
AGACGTG18800.05.68483420-21
CAGACGT15154.7293724E-115.3300918-19
Produced by FastQC (version 0.11.2)